Results: Representative Analyses of Colonies Derived from PCFU (Pancreatic Colony Forming Unit)
8:39
Conclusion
필기록
The overall goal of these protocols is to characterize the cell colonies grown from pancreatic progenitor cells isolated from adult mice using cultures assays that employ methylcellulose containing semi-solid medium. Our methods can help answer ke
이 콘텐츠에 액세스하려면 로그인하거나 무료 평가판을 시작하세요
In vitro colony assays to detect self-renewal and differentiation of progenitor cells isolated from adult murine pancreas are devised. In these assays, pancreatic progenitors give rise to cell colonies in 3-dimensional space in methylcellulose-containing semi-solid medium. Protocols for handling single cells and characterization of individual colonies are described.