Name: multimodal imaging and spectroscopy fiber-bundle microendoscopy platform for non-invasive, in vivo tissue analysis
Uploaded: 2016-10-17T14:00:00.000+00:00
Duration: 10 min 35 s
Description: Watch this Scientific Journal Video about Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis at JoVE.com

This material is based on work supported by the National Institutes of Health (1R03-CA182052, 1R15-CA202662), the National Science Foundation Graduate Research Fellowship Program (G.G., DGE-1450079), the Arkansas Biosciences Institute, and the University of Arkansas Doctoral Academy Fellowship. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the authors and do not necessarily reflect the views of the acknowledged funding agencies.

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The authors declare that they have no competing financial interests.

<html> 멀티 모달 고해상도 이미지 및 여기에보고 서브 확산 반사율 스펙트럼 섬유 다발 microendoscope 최적화 인간 또는 동물 연구 내시경 또는 휴대용 사용을 포함하여 다양한 용도 연구자에 의해 사용될 수있다. 그것은 따라서 두 개의 서로 다른 조직 깊이에서 헤모글로빈 농도, 멜라닌 농도, 조직의 산소 포화도 측정과 함께 생체 내 혀끝의 조직 마이크로 아키텍처의 시각화를위한 유?...

섬유 다발 microendoscopy 기술은 일반적으로 영상 기술 또는 분광 기술들의 조합을 사용하여 생체 조직에서 분석한다. 1-3 그러한 영상 법 고해상도 형광 microendoscopy 서브 셀룰러 해상도 이미지 수 혀끝 조직 미세 구조를 소형으로 , 마이크로 시야, 같은 proflavine, 형광, 또는 pyranine 잉크 등의 국소 조영제를 사용. 1,3-11이 영상 양상이 질적으로 저와 함께 실시간으로 질병과 건강 상피 조직을 차별화 임상 성능을 약속 보여 주었다 간 관찰자 변화. 8 때때로, 연구자는 세포 핵 크기 또는 선 곳은 양적 특징을 추출하는 고해상도 형광 현미경 데이터를 사용하지만이 조직 형태를 시각화 대상으로 주로 질적 기술 남아있다. -1,3,8- 트라이 한편 10 분광 기술, 예컨대확산 반사율 분광학으로, 기능 조직의 정보를 제공하고 정량적으로 여러 장기에 암 병변을 확인하는 임상 성능을 약속 보여 주었다 대상으로한다. 2,12-15 따라서, 잠재적으로, 또한 관찰자 간 변이성을 감소 조직 마이크로 아키텍처의 실시간 시각화를 유지하고, 조직 상태의보다 완전한 분석을 제공하는 양식의 두 가지 유형을 포함하는 장치에 대한 필요성이 존재한다. 이 목표를 달성하기 위해, 복합 프로브 기반 장비가 단일 광섬유 프로브 두 가지 양상 결합한 구성 하였다. 고해상도 형광 microendoscopy 및 서브 확산 반사율 스펙트럼을 정점 정성 고해상도의 화상 (11)이 방법의 공동 레지스터 지역 헤모글로빈 농도를 포함하는 두 개의 별개의 조직 깊이에서 양적 스펙트럼 정보 (기능성)와 조직 형태 (구조적 특성) ([HB는), 멜라닌 농도 ([멜), 및 산소 포화도 (SAO 2). 11,12,16이 특정 하위 확산 반사율 스펙트럼 양상이 제공하는 두 개의 고유 한 조직의 깊이를 샘플링하기 위해 두 개의 소스 검출기 분리 (SDS에)를 사용 기저막 및 기본 조직 기질 아래로 샘플링하여 조직의 건강에 대한 포괄적 인 그림. (11) 파이버 프로브는 약 50,000 4.5 ㎛의 직경의 광섬유 요소, 1.1 mm의 클래드 직경 1.2 mm의 전체 코팅 직경 중앙 1mm 직경 화상 섬유로 구성된다. 이미지 섬유는 220 ㎛, 클래딩 직경이 오 200 μm의 직경 섬유로 둘러싸여 있습니다. 각각 200 ㎛의 다중 모드 광섬유는 거리 화상 광섬유의 중심으로부터 864 ㎛의 중심 간 거리에 위치된다. 200 μm의 멀티 모드 섬유를 각각 25 ° 떨어져있다. &#39;소스&#39;섬유와 왼쪽 200 μm의 멀티 모드 광섬유 및 추가 번째를 사용하여은 &quot;컬렉션&quot;섬유로 200 μm의 멀티 모드 섬유를 REE,이 형상은 반드시 374 μm의 세 가지 중심 간 SDS에, 730 μm의, 1051 μm의 및 1323 μm의를 만듭니다. 섬유 팁은 섬유 상수 사이의 거리를 유지하는 원통형 금속 케이스에 동봉되어 있습니다. 통형 케이싱의 직경은 3mm이다. 광섬유 프로브 (광섬유 프로브 팁을 향해) 말단부 2 피트이다. 프로브는 4피트의 전체 길이에 대해, 추가로 2 피트이다 (계측)을 향해 기단에서 여섯 각 개개의 섬유로 분리한다. (1)는 광섬유 프로브의 표현을 나타낸다. <img alt="그림 1" src="/files/ftp_upload/54564/54564fig1.jpg" /> 그림 1 :. 광섬유 프로브 설계 광섬유 프로브는 하나의 1mm 직경의 이미지 섬유 사 (200) μm의 멀티 모드 섬유로 구성되어 있습니다. 이그림의 표시를 나타낸다 (a) 금속 단부는 SDS의 374, 730를 수득 프로브 팁에서의 섬유의 형상을 제한 캡과 최 좌측 200 μm의 다중 모드 광섬유에 대하여 (눈금 막대 ≈ 1mm)로 1,051 ㎛의, (b) 섬유는 섬유 코어 광섬유 클래딩 및 섬유 피막 (스케일 바 ≈ 1mm), (c)의 섬유가 보호 아미드 외판 (눈금 막대 ≈ 1mm)을 보여주는 금속 캡 내에 제한되고, (d ) 금속 손잡이 모든 섬유를 포함하는 하나의 블랙 케이블 (눈금 막대 ≈ 4mm) 및 (e) 상기 프로브 (눈금 막대 ≈ 4mm의 원위 팁의 화상)와 프로브의 최종 말단 팁. <a href="http://ecsource.jove.com/files/ftp_upload/54564/54564fig1large.jpg" target="_blank">이 그림의 더 큰 버전을 보려면 여기를 클릭하십시오.</a> 이 복합 장비 및 관련 TECHNI다른 결합 구조 / 기능 기술이 서로 다른 양식을 결합하여 존재하지만 가야는 단일 프로브 내에서 이러한 양식의 첫 번째 조합입니다. 예를 들어, 하이퍼 스펙트 럴 영상은 몇 가지 이름을 조직 단백질 발현 분석, (19)와 빛 간섭 단층 촬영 (OCT를) 결합 개발 된 정량적 인 헤모글로빈과 멜라닌 속성, 17, 18 및 다른 기술과 넓은 필드 영상을 결합한 제품입니다. 구강에서의 사용을위한 휴대용 프로브 하부 위장관 식도 또는 내시경 사용을 포함하여 다양한 목적에 최적화 될 수있는 일반적인 광섬유 프로브를 사용하여 컴팩트하고 쉬운 구현 계측 설정이 문서 보고서 외부 피부 배치. 11,20 이 기기의 하드웨어는 확산 반사 스펙트럼을 획득 한 후 결과 있습니다 volum를 추출하는 사용자 정의 데이터 수집 및 후 처리 코드가 모두 필요합니다[혈색소], [멜], 및 상 2를 포함하는 전자 평균 조직 생리 학적 매개 변수를 설정합니다. 사용자 정의 데이터 취득 코드 (고해상도 형광 현미경) 카메라로부터의 동시 획득 및 (확산 반사율 스펙트럼의 경우) 분광계 있도록 지어졌다. 드라이버들은 다양한 프로그래밍 언어와의 통합을 허용하는 제조 업체의 웹 사이트에서 사용할 수 있습니다. 사용자 지정 후 처리 코드는 생체 [혈색소]와 [멜] (21)의 사전 흡수 값을 가져 다음 스펙트럼의 장착 곡선을 생성하는 이전에 개발 된 비선형 최적화 피팅 프로세스를 사용합니다. 22 장착 곡선이을 최소화하여 구축 자체 원료 스펙트럼 사이 χ 2 값 피팅 곡선 및 낮은 χ 2 값. 22 코드로 조직 생체 변수 ([헤모글로빈], [멜, 상투 2)을 결정하는 단계를 포함하도록 수정 될 수있다이러한 여기에 사용되는 외인성 pyranine 잉크로 다른 발색단에서 흡수, 그래서 그 대상 생리 학적 매개 변수는 영향을받지 않습니다. 이러한 [헤모글로빈], [멜, 상투이 같은 조직 상태의 생리 지표는 치료 종양 반응보고 또는 로컬 혈관 형성 및 혈관 신생의 지표로서 사용될 수있다. 14,23을 고해상도 형광 microendoscopy 양상 포함 가이드 프로브 배치를 돕고 상피 조직의 구조 및 기능 사이의 관계의 더욱 완전한 그림으로 연구자를 제공한다. 이 기사, 건설 및 복합 microendoscope의 응용 프로그램에 설명되어 있습니다. (11)

<table><tbody><tr><td>30 mm Cage Cube with Dichroic Filter Mount</td><td>Thorlabs, Inc.</td><td>CM1-DCH</td><td></td></tr><tr><td>470 nm Dichroic Mirror (Beam Splitter)</td><td>Chroma Corporation</td><td>T470lpxr</td><td></td></tr><tr><td>Cage Assembly Rod, 1.5&quot;, 4-Pack</td><td>Thorlabs, Inc.</td><td>ER1.5-P4</td><td></td></tr><tr><td>Cage Assembly Rod, 3.0&quot;, 4-Pack</td><td>Thorlabs, Inc.</td><td>ER3-P4</td><td></td></tr><tr><td>Cage Assembly Rod, 2.0&quot;, 4-Pack</td><td>Thorlabs, Inc.</td><td>ER2-P4</td><td></td></tr><tr><td>SM1-Threaded 30 mm Cage Plate</td><td>Thorlabs, Inc.</td><td>CP02</td><td></td></tr><tr><td>SM1 Series Stress-Free Retaining Ring</td><td>Thorlabs, Inc.</td><td>SM1PRR</td><td></td></tr><tr><td>SM1 Lens Tube, 1.00&quot; Thread Depth</td><td>Thorlabs, Inc.</td><td>SM1L10</td><td></td></tr><tr><td>Right-Angle Kinematic Mirror Mount</td><td>Thorlabs, Inc.</td><td>KCB1</td><td></td></tr><tr><td>1&quot; UV Enhanced Aluminum Mirror</td><td>Thorlabs, Inc.</td><td>PF10-03-F01</td><td></td></tr><tr><td>Z-Axis Translation Mount</td><td>Thorlabs, Inc.</td><td>SM1Z</td><td></td></tr><tr><td>10X Olympus Plan Achromatic Objective</td><td>Thorlabs, Inc.</td><td>RMS10X</td><td></td></tr><tr><td>XY Translating Lens Mount</td><td>Thorlabs, Inc.</td><td>CXY1</td><td></td></tr><tr><td>SMA Fiber Adapter Plate with SM1 Thread</td><td>Thorlabs, Inc.</td><td>SM1SMA</td><td></td></tr><tr><td>SM1 Lens Tube, 0.50&quot; Thread Depth</td><td>Thorlabs, Inc.</td><td>SM1L05</td><td></td></tr><tr><td>440/40 Bandpass Filter (Excitation)</td><td>Chroma Corporation</td><td>ET440/40x</td><td></td></tr><tr><td>525/36 Bandpass Filter (Emission)</td><td>Chroma Corporation</td><td>ET525/36m</td><td></td></tr><tr><td>Quick Set Epoxy</td><td>Loctite</td><td>1395391</td><td></td></tr><tr><td>455 nm LED Light Housing Kit - 3-Watt</td><td>LED Supply</td><td>ALK-LH-3W-KIT</td><td></td></tr><tr><td>1&quot; Achromatic Doublet, f = 50 mm</td><td>Thorlabs, Inc.</td><td>AC254-050-A</td><td></td></tr><tr><td>Flea 3 USB Monochrome Camera</td><td>Point Grey, Inc.</td><td>FL3-U3-32S2M-CS</td><td></td></tr><tr><td>0.5&quot; Post Holder, L = 1.5&quot;</td><td>Thorlabs, Inc.</td><td>PH1.5</td><td></td></tr><tr><td>0.5&quot; Optical Post, L = 4.0&quot;</td><td>Thorlabs, Inc.</td><td>TR4</td><td></td></tr><tr><td>Mounting Base, 1&quot; x 2.3&quot; x 3/8&quot;</td><td>Thorlabs, Inc.</td><td>BA1S</td><td></td></tr><tr><td>Long Lifetime Tungsten-Halogen Light Source (Vis-NIR)</td><td>Ocean Optics</td><td>HL-2000-LL</td><td></td></tr><tr><td>20X Olympus Plan Objective</td><td>Edmund Optics, Inc.</td><td>PLN20X</td><td></td></tr><tr><td>Custom-Built Aluminum Motor Arm</td><td>N/A</td><td>N/A</td><td>Custom designed and built</td></tr><tr><td>Custom-Built Aluminum Motor Arm Adaptor</td><td>N/A</td><td>N/A</td><td>Custom designed and built</td></tr><tr><td>Custom-Built Aluminum Motor Housing</td><td>N/A</td><td>N/A</td><td>Custom designed and built</td></tr><tr><td>Stepper Motor - 400 steps/revolution</td><td>SparkFun Electronics</td><td>ROB-10846</td><td>Multiple suppliers</td></tr><tr><td>Custom-Built Aluminum Optical Fiber Switch</td><td>N/A</td><td>N/A</td><td>Custom designed and built</td></tr><tr><td>Custom-Built Aluminum Optical Fiber Switch Face-Plate</td><td>N/A</td><td>N/A</td><td>Custom designed and built</td></tr><tr><td>Arduino Uno - R3</td><td>SparkFun Electronics</td><td>DEV-11021</td><td>Multiple suppliers</td></tr><tr><td>Electronic Breadboard - Self-Adhesive</td><td>SparkFun Electronics</td><td>PRT-12002</td><td>Multiple suppliers</td></tr><tr><td>EasyDriver - Stepper Motor Driver</td><td>Sparkfun Electronics</td><td>ROB-12779</td><td></td></tr><tr><td>12 V, 229 mA Power Supply</td><td>Phihong</td><td>PSM03A</td><td>Multiple suppliers</td></tr><tr><td>Enhanced Sensitivity USB Spectrometer (Vis-NIR)</td><td>Ocean Optics</td><td>USB2000+VIS-NIR-ES</td><td></td></tr><tr><td>550 &amp;micro;m, 0.22 NA, SMA-SMA Fiber Patch Cable</td><td>Thorlabs, Inc.</td><td>M37L01</td><td></td></tr><tr><td>Custom-Built Fiber-Optic Probe</td><td>Myriad Fiber Imaging</td><td>N/A</td><td></td></tr><tr><td>20% Spectralon Diffuse Reflectance Standard</td><td>Labsphere, Inc.</td><td>SRS-20-010</td><td></td></tr><tr><td>Standard Yellow Highlighter</td><td>Sharpie</td><td>25005</td><td>Multiple suppliers, proflavine or fluorescein can be substituted</td></tr></tbody></table>

multimodal imaging and spectroscopy fiber-bundle microendoscopy platform for non-invasive, in vivo tissue analysis

최근 섬유 다발 microendoscopy 기술 이미징 기술 또는 분광 기술들의 조합을 사용하여 생체 내 조직의 비파괴 분석을 가능하게한다. 하나의 광학 탐침으로 이미징 분광기 기술을 결합하여 조직 상태의보다 완전한 분석을 제공 할 수있다. 이 기사에서는 두 개의 서로 다른 양식은 하나의 광학 프로브로, 고해상도 형광 microendoscopy 이미징 및 확산 반사율 분광학을 결합됩니다. 고해상도 형광 microendoscopy 촬상 주로 질적 기술되지만, 종양 및 비 종양 조직 간의 효율적인 실시간 분화를 보여 주었다, 정점 조직 미세 구조를 시각화하기 위해 사용되는 기술이며. 확산 반사율 스펙트럼은 로컬 헤모글로빈 농도 멜라닌 농도, 산소 포화도를 포함한 조직 생리 학적 파라미터를 추출 할 수있는 기술이다. 이 글은 사양 r에 대해 설명계측을 구축하고 생체 인간의 피부에 기술을 보여줍니다하는 방법, 광섬유 프로브를 구성하는 equired. 이 작품은 조직 마이크로 아키텍처, 특히 혀끝의 피부 각질 세포가, 그와 관련된 생리 학적 매개 변수와 함께 공동 등록 할 수 있습니다 것으로 나타났습니다. 여기에 제시된 수단 및 섬유 다발 프로브 장기 다양한 시스템에서 사용하기위한 핸드 헬드 또는 내시경 호환 장치 중 하나로 최적화 될 수있다. 추가의 임상 연구는 상이한 상피 질환 상태에 대한 이러한 기술의 가능성을 테스트하기 위해 필요하다.

이 프로토콜에 따라, 연구자는 뷰의 전체 필드 (도 5)로 조직 부위의 포커스에 고해상도 이미지를 얻을 것이다. 표준 노란색 형광펜에서 pyranine 잉크로 염색하는 경우 등 proflavine 등의 염료로 염색하는 경우 각각의 세포 핵을 볼 수있는 반면, 세포의 개요는, 볼 수 있습니다. 스펙트럼 수집 후, 후 처리 소프트웨어 서브 확산 반사율 스펙트럼을 맞고 [헤모글로빈...

Watch this Scientific Journal Video about Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis at JoVE.com

Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis

The assembly and use of a multimodal microendoscope is described which can co-register superficial tissue image data with tissue physiological parameters including hemoglobin concentration, melanin concentration, and oxygen saturation. This technique can be useful for evaluating tissue structure and perfusion, and can be optimized for individual needs of the investigator.

멀티 모달 이미징 및 비 침습적에 대한 분광학 섬유 다발 Microendoscopy 플랫폼,<em&gt; 생체</em&gt; 조직 분석

Recent fiber-bundle microendoscopy techniques enable non-invasive analysis of in vivo tissue using either imaging techniques or a combination of ...

multimodal-imaging-spectroscopy-fiber-bundle-microendoscopy-platform

Research

JoVE Journal

Bioengineering

Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis

7.8K 조회수.  University of Arkansas. The assembly and use of a multimodal microendoscope is described which can co-register superficial tissue image data with tissue physiological parameters including hemoglobin concentration, melanin concentration, and oxygen saturation. This technique can be useful for evaluating tissue structure and perfusion, and can be optimized for individual needs of the investigator.

비디오: Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis

High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging

Many biological and clinical studies require the longitudinal study and analysis of morphology and function with cellular level resolution. Traditionally, multiple experiments are run in parallel, with individual samples removed from the study at sequential time points for evaluation by light microscopy. Several intravital techniques have been developed, with confocal, multiphoton, and second harmonic microscopy all demonstrating their ability to be used for imaging in situ 1. With these systems, however, the required infrastructure is complex and expensive, involving scanning laser systems and complex light sources. Here we present a protocol for the design and assembly of a high-resolution microendoscope which can be built in a day using off-the-shelf components for under US$5,000. The platform offers flexibility in terms of image resolution, field-of-view, and operating wavelength, and we describe how these parameters can be easily modified to meet the specific needs of the end user.
We and others have explored the use of the high-resolution microendoscope (HRME) in in vitro cell culture 2-5, in excised 6 and living animal tissues 2,5, and in human tissues in vivo 2,7. Users have reported the use of several different fluorescent contrast agents, including proflavine 2-4, benzoporphyrin-derivative monoacid ring A (BPD-MA) 5, and fluoroscein 6,7, all of which have received full, or investigational approval from the FDA for use in human subjects. High-resolution microendoscopy, in the form described here, may appeal to a wide range of researchers working in the basic and clinical sciences. The technique offers an effective and economical approach which complements traditional benchtop microscopy, by enabling the user to perform high-resolution, longitudinal imaging in situ.

High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging

In many biological and clinical situations it is advantageous to study cellular processes as they evolve in their native microenvironment. Here we describe the assembly and use of a low-cost fiber-optic microscope which can provide real time imaging in cell culture, animal studies, and clinical patient studies.

고해상도 광섬유 Microendoscopy에 대한 현장에서 세포 이미징

Many biological and clinical studies require the longitudinal study and analysis of morphology and function with cellular level resolution.  ...

연구

생체공학

Dual-mode Imaging of Cutaneous Tissue Oxygenation and Vascular Function

Accurate assessment of cutaneous tissue oxygenation and vascular function is important for appropriate detection, staging, and treatment of many health disorders such as chronic wounds. We report the development of a dual-mode imaging system for non-invasive and non-contact imaging of cutaneous tissue oxygenation and vascular function. The imaging system integrated an infrared camera, a CCD camera, a liquid crystal tunable filter and a high intensity fiber light source. A Labview interface was programmed for equipment control, synchronization, image acquisition, processing, and visualization. Multispectral images captured by the CCD camera were used to reconstruct the tissue oxygenation map. Dynamic thermographic images captured by the infrared camera were used to reconstruct the vascular function map. Cutaneous tissue oxygenation and vascular function images were co-registered through fiduciary markers. The performance characteristics of the dual-mode image system were tested in humans.

A dual-mode imaging system was developed for non-contact assessment of cutaneous tissue oxygenation and vascular function.

피부 조직 산소와 혈관 기능의 듀얼 모드 영상

Accurate assessment of cutaneous tissue oxygenation and vascular function is important for appropriate detection, staging, and treatment of many health ...

의학

Universal Hand-held Three-dimensional Optoacoustic Imaging Probe for Deep Tissue Human Angiography and Functional Preclinical Studies in Real Time

The exclusive combination of high optical contrast and excellent spatial resolution makes optoacoustics (photoacoustics) ideal for simultaneously attaining anatomical, functional and molecular contrast in deep optically opaque tissues. While enormous potential has been recently demonstrated in the application of optoacoustics for small animal research, vast efforts have also been undertaken in translating this imaging technology into clinical practice. We present here a newly developed optoacoustic tomography approach capable of delivering high resolution and spectrally enriched volumetric images of tissue morphology and function in real time. A detailed description of the experimental protocol for operating with the imaging system in both hand-held and stationary modes is provided and showcased for different potential scenarios involving functional and molecular studies in murine models and humans. The possibility for real time visualization in three dimensions along with the versatile handheld design of the imaging probe make the newly developed approach unique among the pantheon of imaging modalities used in today&#8217;s preclinical research and clinical practice.

We provide herein a detailed description of the experimental protocol for imaging with a newly developed hand-held optoacoustic (photoacoustic) system for three-dimensional functional and molecular imaging in real time. The demonstrated powerful performance and versatility may define new application areas of the optoacoustic technology in preclinical research and clinical practice.

범용 실시간으로 깊은 조직 인간의 혈관 조영술과 기능 전임상 연구를위한 3 차원 광 음향 이미징 프로브를 손 개최

The exclusive combination of high optical contrast and excellent spatial resolution makes optoacoustics (photoacoustics) ideal for simultaneously ...

Three-Dimensional Ultrasonic Needle Tip Tracking with a Fiber-Optic Ultrasound Receiver

Ultrasound is frequently used for guiding minimally invasive procedures, but visualizing medical devices is often challenging with this imaging modality. When visualization is lost, the medical device can cause trauma to critical tissue structures. Here, a method to track the needle tip during ultrasound image-guided procedures is presented. This method involves the use of a fiber-optic ultrasound receiver that is affixed within the cannula of a medical needle to communicate ultrasonically with the external ultrasound probe. This custom probe comprises a central transducer element array and side element arrays. In addition to conventional two-dimensional (2D) B-mode ultrasound imaging provided by the central array, three-dimensional (3D) needle tip tracking is provided by the side arrays. For B-mode ultrasound imaging, a standard transmit-receive sequence with electronic beamforming is performed. For ultrasonic tracking, Golay-coded ultrasound transmissions from the 4 side arrays are received by the hydrophone sensor, and subsequently the received signals are decoded to identify the needle tip&#39;s spatial location with respect to the ultrasound imaging probe. As a preliminary validation of this method, insertions of the needle/hydrophone pair were performed in clinically realistic contexts. This novel ultrasound imaging/tracking method is compatible with current clinical workflow, and it provides reliable device tracking during in-plane and out-of-plane needle insertions.

Accurate and efficient visualization of invasive medical devices is extremely important in many ultrasound-guided minimally invasive procedures. Here, a method for localizing the spatial position of a needle tip relative to the ultrasound imaging probe is presented.

3 차원 초음파 바늘 팁 광섬유 초음파 수신기와 함께 추적

Ultrasound is frequently used for guiding minimally invasive procedures, but visualizing medical devices is often challenging with this imaging modality. ...

공학

Murine Endoscopy for In Vivo Multimodal Imaging of Carcinogenesis and Assessment of Intestinal Wound Healing and Inflammation

Mouse models are widely used to study pathogenesis of human diseases and to evaluate diagnostic procedures as well as therapeutic interventions preclinically. However, valid assessment of pathological alterations often requires histological analysis, and when performed ex vivo, necessitates death of the animal. Therefore in conventional experimental settings, intra-individual follow-up examinations are rarely possible. Thus, development of murine endoscopy in live mice enables investigators for the first time to both directly visualize the gastrointestinal mucosa and also repeat the procedure to monitor for alterations. Numerous applications for in vivo murine endoscopy exist, including studying intestinal inflammation or wound healing, obtaining mucosal biopsies repeatedly, and to locally administer diagnostic or therapeutic agents using miniature injection catheters. Most recently, molecular imaging has extended diagnostic imaging modalities allowing specific detection of distinct target molecules using specific photoprobes. In conclusion, murine endoscopy has emerged as a novel cutting-edge technology for diagnostic experimental in vivo imaging and may significantly impact on preclinical research in various fields.

Murine Endoscopy for In Vivo Multimodal Imaging of Carcinogenesis and Assessment of Intestinal Wound Healing and Inflammation

Small animal imaging techniques allow serial diagnostic examinations and therapeutic interventions in vivo. Recently, the scope of applications has significantly widened and currently includes assessment of colonic tumor development, wound healing and monitoring of inflammation. This protocol illustrates these diverse potential applications of murine endoscopy.

에 대한 쥐의 내시경<em&gt; 생체</em&gt; 발암 및 소장의 평가의 멀티 모달 이미징 상처 치유 및 염증

Mouse models are widely used to study pathogenesis of human diseases and to evaluate diagnostic procedures as well as therapeutic interventions ...

VSFG 현미경을 사용하여 다중 모드, 신속, 초분광 화학 분석

Multimodal Nonlinear Hyperspectral Chemical Imaging Using Line-Scanning Vibrational Sum-Frequency Generation Microscopy

Vibrational sum-frequency generation (VSFG), a second-order nonlinear optical signal, has traditionally been used to study molecules at interfaces as a spectroscopy technique with a spatial resolution of ~100 &#181;m. However, the spectroscopy is not sensitive to the heterogeneity of a sample. To study mesoscopically heterogeneous samples, we, along with others, pushed the resolution limit of VSFG spectroscopy down to ~1 &#181;m level and constructed the VSFG microscope. This imaging technique not only can resolve sample morphologies through imaging, but also record a broadband VSFG spectrum at every pixel of the images. Being a second-order nonlinear optical technique, its selection rule enables the visualization of non-centrosymmetric or chiral self-assembled structures commonly found in biology, materials science, and bioengineering, among others. In this article, the audience will be guided through an inverted transmission design that allows for imaging unfixed samples. This work also showcases that VSFG microscopy can resolve chemical-specific geometric information of individual self-assembled sheets by combining it with a neural network function solver. Lastly, the images obtained under brightfield, SHG, and VSFG configurations of various samples briefly discuss the unique information revealed by VSFG imaging.

저자 스포트라이트: Mesoscopically Heterogeneous Self-Assembled Structures 연구에서 VSFG 현미경의 잠재력 공개 

A multimodal, rapid hyperspectral imaging framework was developed to obtain broadband vibrational sum-frequency generation (VSFG) images, along with brightfield, second harmonic generation (SHG) imaging modalities. Due to the infrared frequency being resonant with molecular vibrations, microscopic structural and mesoscopic morphology knowledge is revealed of symmetry-allowed samples.

라인 스캐닝 진동 합계-주파수 생성 현미경을 사용한 다중 모드 비선형 초분광 화학 이미징

Vibrational sum-frequency generation (VSFG), a second-order nonlinear optical signal, has traditionally been used to study molecules at interfaces as a ...

화학

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

In cardiac muscle, intracellular Ca2+ transients activate contractile myofilaments, causing contraction, macroscopic shortening, and geometric&#160;deformation. Our understanding of the internal relationships between these events has been limited because we can neither &#39;see&#39; inside the muscle nor precisely track the spatio-temporal nature of excitation-contraction dynamics. To resolve these problems, we have constructed a device that combines a suite of imaging modalities. Specifically, it integrates a brightfield microscope to measure local changes of sarcomere length and tissue strain, a fluorescence microscope to visualize the Ca2+ transient, and an optical coherence tomograph to capture the tissue&#39;s geometric&#160;changes throughout the time-course of a cardiac cycle. We present here the imaging infrastructure and associated data collection framework. Data are collected from isolated rod-like tissue structures known as trabeculae carneae. In our instrument, a pair of position-controlled platinum hooks hold each end of an ex vivo muscle sample while it is continuously superfused with nutrient-rich saline solution. The hooks are under independent control, permitting real-time control of muscle length and force. Lengthwise translation enables the piecewise scanning of the sample, overcoming limitations associated with the relative size of the microscope imaging window (540 &#181;m by 540 &#181;m) and the length of a typical trabecula (&#62;2000 &#181;m). Platinum electrodes at either end of the muscle chamber stimulate the trabecula at a user-defined rate. We exploit the stimulation signal as a trigger for synchronizing the data from each imaging window to reconstruct the entire sample twitching under steady-state conditions. Applying image-processing techniques to these brightfield imaging data provides tissue displacement and sarcomere length maps. Such a collection of data, when incorporated into an experiment-modeling pipeline, will provide a deeper understanding of muscle contractile homogeneity and heterogeneity in physiology and pathophysiology.

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

This protocol presents a collection of sarcomere, calcium, and macroscopic geometric&#160;data from an actively contracting cardiac trabecula ex vivo. These simultaneous measurements are made possible by the integration of three imaging modalities.

동시 브라이트필드, 형광, 및 수축 심장 Trabeculae Ex Vivo의 광학 일관성 지형 이미징

In cardiac muscle, intracellular Ca2+ transients activate contractile myofilaments, causing contraction, macroscopic shortening, and ...

In vivo Imaging of Biological Tissues with Combined Two-Photon Fluorescence and Stimulated Raman Scattering Microscopy

Stimulated Raman scattering (SRS) microscopy enables label-free imaging of the biological tissues in its natural microenvironment based on intrinsic molecular vibration, thus providing a perfect tool for in vivo study of biological processes at subcellular resolution. By integrating two-photon excited fluorescence (TPEF) imaging into the SRS microscope, the dual-modal in vivo imaging of tissues can acquire critical biochemical and biophysical information from multiple perspectives which helps understand the dynamic processes involved in cellular metabolism, immune response and tissue remodeling, etc. In this video protocol, the setup of a TPEF-SRS microscope system as well as the in vivo imaging method of the animal spinal cord is introduced. The spinal cord, as part of the central nervous system, plays a critical role in the communication between the brain and peripheral nervous system. Myelin sheath, abundant in phospholipids, surrounds and insulates the axon to permit saltatory conduction of action potentials. In vivo imaging of myelin sheaths in the spinal cord is important to study the progression of neurodegenerative diseases and spinal cord injury. The protocol also describes animal preparation and in vivo TPEF-SRS imaging methods to acquire high-resolution biological images.

In vivo Imaging of Biological Tissues with Combined Two-Photon Fluorescence and Stimulated Raman Scattering Microscopy

Stimulated Raman scattering (SRS) microscopy allows label-free imaging of biomolecules based on their intrinsic vibration of specific chemical bonds. In this protocol, the instrumental setup of an integrated SRS and two-photon fluorescence microscope is described to visualize cellular structures in the spinal cord of live mice.

생체 내 결합 된 이광자 형광 및 자극 된 라만 산란 현미경을 사용한 생물학적 조직 이미징

Stimulated Raman scattering (SRS) microscopy enables label-free imaging of the biological tissues in its natural microenvironment based on intrinsic ...

멀티 모달 이미징 및 비 침습적에 대한 분광학 섬유 다발 Microendoscopy 플랫폼,

요약

더 많은 비디오 탐색

이 비디오의 챕터

고해상도 광섬유 Microendoscopy에 대한 현장에서 세포 이미징

피부 조직 산소와 혈관 기능의 듀얼 모드 영상

범용 실시간으로 깊은 조직 인간의 혈관 조영술과 기능 전임상 연구를위한 3 차원 광 음향 이미징 프로브를 손 개최

3 차원 초음파 바늘 팁 광섬유 초음파 수신기와 함께 추적

에 대한 쥐의 내시경

라인 스캐닝 진동 합계-주파수 생성 현미경을 사용한 다중 모드 비선형 초분광 화학 이미징

라인 스캐닝 진동 합계-주파수 생성 현미경을 사용한 다중 모드 비선형 초분광 화학 이미징

라인 스캐닝 진동 합계-주파수 생성 현미경을 사용한 다중 모드 비선형 초분광 화학 이미징

동시 브라이트필드, 형광, 및 수축 심장 Trabeculae Ex Vivo의 광학 일관성 지형 이미징

생체 내 결합 된 이광자 형광 및 자극 된 라만 산란 현미경을 사용한 생물학적 조직 이미징