Kidney, Aorta, and Lymph Node Isolation and Single Cell Suspension Generation
4:29
Ex Vivo Stimulation and Intracellular Cytokine Detection
7:07
Results: Representative Analyses of Kidney, Aorta, and Aortic Lymph Node T Cell Activation
8:23
Conclusion
필기록
The overall goal of this protocol is to obtain single-cell suspensions from the murine aorta, kidney, and lymph nodes to quantify and characterize immune cells in a model of angiotensin II-induced hypertension. This method can help answer key ques
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This article provides detailed methodology to identify and quantify functional T lymphocyte subsets present within murine kidney, aorta and lymph nodes by intracellular staining and flow cytometry. The model of angiotensin II induced hypertension was chosen to explain, step-by-step, the procedures and fundamental principles of flow cytometry and intracellular staining.