The overall goal of this tick-feeding protocol is to improve the existing standards by increasing the survival of blood-fed ticks, while reducing the stress to the rodent host. In addition to simplifying tick colony rearing, this method provides a
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The production of healthy laboratory-reared ticks is essential to studies on tick biology, and tick-pathogen interactions. Here we demonstrate a simple protocol for immature tick feeding that is cost-effective and less stressful to mice.