Synthesis of ManNProp and Related N-acyl-modefied Mannosamines
2:26
Cell Culture and Cell Lysis for HPLC Analysis
4:28
Separation of the Membrane Fraction and Acid Hydrolysis
5:27
Fluorescent Labeling, HPLC, and Mass Spetrometry Analysis
6:55
Western Blot Analysis of Polysialic Acid in Kelly Neuroblastoma Cells
9:00
Results: Modulating Expression of Sialic Acid on Cell Surfaces Using N-Side Chain Modified Mannosamines
10:40
Conclusion
필기록
The overall goal of this technique is to generate cells or cell derived products with modified sialic acid expression. Therefore, cells are treated with N-acyl modified Mannosamines which are metabolized to the corresponding sialic acids and subse
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Sialic acid is a typical monosaccharide-unit found in glycoconjugates. It is involved in a plethora of molecular and cellular interactions. Here we present a method to modify cell surface sialic acid expression using metabolic glycoengineering with N-acetylmannosamine derivatives.