Name: construction and evaluation of a murine calvarial osteolysis model by exposure to cocrmo particles in aseptic loosening
Uploaded: 2018-02-17T14:00:00
Description: Watch this Scientific Journal Video about Construction and Evaluation of a Murine Calvarial Osteolysis Model by Exposure to CoCrMo Particles in Aseptic Loosening at JoVE.com

This study was supported by the National Natural Science Foundation of China (81572111), the Clinical Science and Technology Project Foundation of Jiangsu Province (BL2012002), the Scientific Research Project of Nanjing (201402007), the Natural Science Foundation of Jiangsu Province (BK20161385), and the Special Foundation of Chinese Medical Doctor Association (2015COS0810).

All methods described here have been approved by the Institutional Animal Care and Use Committee (IACUC) of Nanjing University.
1. CoCrMo Particle Preparation
<ol>
	<li>Obtain CoCrMo particles by using a fabricated high-vacuum three-electrode direct current4. Place CoCrMo alloy in the instrument under 10-3 Pa vacuum, 0.04 MPa argon and hydrogen 3:2 (v/v), and 650 A cathode current.</li>
	<li>Measure the diameters of CoCrMo particles.
	<ol>
		<li>Add 1 mg of...

<ol>
	<li>Dong, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Antisense+oligonucleotide+targeting+TNF-alpha+can+suppress+Co-Cr-Mo+particle-induced+osteolysis.">Antisense oligonucleotide targeting TNF-alpha can suppress Co-Cr-Mo particle-induced osteolysis.</a> J Orthop Res. 26 (8), 1114-1120 (2008).</li><li>Deng, Z., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=SIRT1+protects+osteoblasts+against+particle-induced+inflammatory+responses+and+apoptosis+in+aseptic+prosthesis+loosening.">SIRT1 protects osteoblasts against particle-induced inflammatory responses and apoptosis in aseptic prosthesis loosening.</a> Acta Biomater. 49, 541-554 (2017).</li><li>Langlois, J., Hamadouche, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=New+animal+models+of+wear-particle+osteolysis.">New animal models of wear-particle osteolysis.</a> Int Orthop. 35 (2), 245-251 (2011).</li><li>Wang, P., Zhao, F. X., Zhang, Z. Z. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Preparation+of+ultrafine+zinc+powders+by+DC+arc+plasma+evaporation+method.">Preparation of ultrafine zinc powders by DC arc plasma evaporation method.</a> Chinese Journal of Nonferrous Metals. 21 (9), 2236-2241 (2011).</li><li>Wang, Z., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+fibroblast+expression+of+RANKL+in+CoCrMo-particle-induced+osteolysis+is+mediated+by+ER+stress+and+XBP1s.">The fibroblast expression of RANKL in CoCrMo-particle-induced osteolysis is mediated by ER stress and XBP1s.</a> Acta Biomater. 24, 352-360 (2015).</li><li>Wang, Z., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Autophagy+mediated+CoCrMo+particle-induced+peri-implant+osteolysis+by+promoting+osteoblast+apoptosis.">Autophagy mediated CoCrMo particle-induced peri-implant osteolysis by promoting osteoblast apoptosis.</a> Autophagy. 11 (12), 2358-2369 (2015).</li><li>Wang, R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Particle-induced+osteolysis+mediated+by+endoplasmic+reticulum+stress+in+prosthesis+loosening.">Particle-induced osteolysis mediated by endoplasmic reticulum stress in prosthesis loosening.</a> Biomaterials. 34 (11), 2611-2623 (2013).</li><li>Yang, S. Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Adeno-associated+virus-mediated+osteoprotegerin+gene+transfer+protects+against+particulate+polyethylene-induced+osteolysis+in+a+murine+model.">Adeno-associated virus-mediated osteoprotegerin gene transfer protects against particulate polyethylene-induced osteolysis in a murine model.</a> Arthritis Rheum. 46 (9), 2514-2523 (2002).</li><li>Liu, N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Autophagy+mediated+TiAl(6)V(4)+particle-induced+peri-implant+osteolysis+by+promoting+expression+of+TNF-alpha.">Autophagy mediated TiAl(6)V(4) particle-induced peri-implant osteolysis by promoting expression of TNF-alpha.</a> Biochem Biophys Res Commun. 473 (1), 133-139 (2016).</li><li>Wang, Z., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=ER+Stress+Mediates+TiAl6V4+Particle-Induced+Peri-Implant+Osteolysis+by+Promoting+RANKL+Expression+in+Fibroblasts.">ER Stress Mediates TiAl6V4 Particle-Induced Peri-Implant Osteolysis by Promoting RANKL Expression in Fibroblasts.</a> PLoS One. 10 (9), e0137774 (2015).</li><li>Wang, Z., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=TiAl6V4+particles+promote+osteoclast+formation+via+autophagy-mediated+downregulation+of+interferon-beta+in+osteocytes.">TiAl6V4 particles promote osteoclast formation via autophagy-mediated downregulation of interferon-beta in osteocytes.</a> Acta Biomater. 48, 489-498 (2017).</li><li>Chen, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Lycorine+suppresses+RANKL-induced+osteoclastogenesis+in+vitro+and+prevents+ovariectomy-induced+osteoporosis+and+titanium+particle-induced+osteolysis+in+vivo.">Lycorine suppresses RANKL-induced osteoclastogenesis in vitro and prevents ovariectomy-induced osteoporosis and titanium particle-induced osteolysis in vivo.</a> Sci Rep. 5, 12853 (2015).</li><li>Neuerburg, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+role+of+calcitonin+receptor+signalling+in+polyethylene+particle-induced+osteolysis.">The role of calcitonin receptor signalling in polyethylene particle-induced osteolysis.</a> Acta Biomater. 14, 125-132 (2015).</li><li>Catelas, I., Jacobs, J. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Biologic+activity+of+wear+particles.">Biologic activity of wear particles.</a> Instr Course Lect. 59, 3-16 (2010).</li><li>Liu, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+biological+response+to+nanometre-sized+polymer+particles.">The biological response to nanometre-sized polymer particles.</a> Acta Biomater. 23, 38-51 (2015).</li></ol>

There are two main methods for wear particle-induced osteolysis in mice: the air-pouch model and the calvarial osteolysis model. In the air-pouch model, a subcutaneously generated air-pouch is first established, followed by wear particle introduction and implantation into the bone tissue8. The pouch wall mimics the periosteum in aseptic loosening. However, bone implantation is nonvascular with no biological activity, which makes it difficult to assess direct interactions between particles and the ...

Aseptic loosening is the most common cause of total hip arthroplasty (THA) and total knee arthroplasty (TKA) failure, which requires revision surgery1. However, the underlying mechanism remains unclear2. A long follow-up is required to detect particle-induced osteolysis, whose occurrence is rare; therefore, it is challenging to explore its pathogenesis in clinical cases. Hence, further studies focusing on complex cellular and tissue mechanisms require both in vivo experiments in wear particle-induced osteolysis models and in vitro assays in cells related to bone homeostasis3. A valid animal model is important in revealing the effects of wear particles on bone loss, providing evidence for further cellular assays. 
A murine calvarial osteolysis model constructed by exposure to CoCrMo particles is an effective and valid method for assessing the interactions between particles and various cells in aseptic loosening. In this model, CoCrMo particles cause calvarial osteolysis by inducing inflammatory cytokines in macrophages, activating osteoclasts, inhibiting osteoblast proliferation, and promoting osteoblast apoptosis.
It only takes two weeks to establish this model. Osteolysis can be visualized and quantified by hematoxylin and eosin (H&amp;E) staining and micro computed tomography (micro-CT)2. In addition, this model has a relatively low cost, and transgenic and knockout mouse models can be used to screen a large number of compounds at various doses3.
The procedure to establish and evaluate this model is simple. First, CoCrMo particles were obtained by high-vacuum three-electrode direct current and resuspended in phosphate-buffered saline (PBS) at a concentration of 50 mg/mL. Then, 50 &#181;L of the resulting suspension was applied to the middle of the murine calvaria after separation of the cranial periosteum by sharp dissection. The mice were sacrificed after two weeks, and calvaria samples were harvested; qualitative and quantitative analyses were performed by H&amp;E staining andmicro-CT. 
A murine calvarial osteolysis model constructed by exposure to CoCrMo particles is an ideal tool for assessing the interactions between CoCrMo particles and various cells, such as macrophages, fibroblasts, osteoblasts, and osteoclasts, in aseptic loosening.

<table border="0" cellpadding="0" cellspacing="0" width="684">
	<tbody>
		<tr height="26">
			<td height="26" style="height:26px;width:216px;">CoCrMo alloy from prosthesis</td>
			<td style="width:183px;">Waldemar Link GmbH &#38; Co</td>
			<td style="width:119px;">GEMINI MK II</td>
			<td style="width:167px;">Raw material to obtain CoCrMo nanoparticles</td>
		</tr>
		<tr height="84">
			<td height="84" style="height:84px;width:216px;">Fabricated high-vacuum three-electrode direct current</td>
			<td style="width:183px;">College of Materials Science &#38; Engineering , Nanjing University of Technology</td>
			<td style="width:119px;"></td>
			<td>Self designed machine</td>
		</tr>
		<tr height="61">
			<td height="61" style="height:61px;width:216px;">6 week old male C57BL/6J mice</td>
			<td style="width:183px;">Model animal research center of Nanjing University</td>
			<td style="width:119px;">N000013</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">100% Ethanol</td>
			<td style="width:183px;">Nanjing Reagent</td>
			<td>C0691514023</td>
			<td>Solvent of CoCrMo nanoparticles for transmission electron microscope scanning</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">1.5 ml Microcentrifuge tubes</td>
			<td style="width:183px;">Taizhou Weierkang Medical Supplies co., LTD</td>
			<td>W603</td>
			<td></td>
		</tr>
		<tr height="63">
			<td height="63" style="height:63px;width:216px;">Microanalytical balance</td>
			<td style="width:183px;">Shenzhen Qun long Instrument Equipment Co,. LTD</td>
			<td>EX125DZH</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Ultrasonic shaker</td>
			<td style="width:183px;">Shanghai Yuhao scientific instrument co., LTD</td>
			<td style="width:119px;">YH-200DH</td>
			<td>To suspend CoCrMo nanoparticles</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Transmission Electron Microscope</td>
			<td style="width:183px;">FEI</td>
			<td style="width:119px;">Tecnai G20</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">SimplePCI software</td>
			<td style="width:183px;">Compix Inc.</td>
			<td style="width:119px;">6.6 version</td>
			<td>To calculate the mean diameter and particle size distribution.</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">High-handed sterilization pan</td>
			<td style="width:183px;">QIULONGYIQI</td>
			<td style="width:119px;">KYQL-100DS</td>
			<td>To decontaminate endotoxin</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Limulus Amebocyte Lysate (LAL) Assay</td>
			<td style="width:183px;">Charles River</td>
			<td style="width:119px;">R13025</td>
			<td>To detect endotoxin&#160;</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">15 ml Microcentrifuge tubes</td>
			<td style="width:183px;">Taizhou Suyi Medical</td>
			<td style="width:119px;">B122</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Phosphate-buffered saline</td>
			<td style="width:183px;">Boster Biological Technology</td>
			<td style="width:119px;">AR0030</td>
			<td>Solvent of CoCrMo nanoparticles stock solution</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Pentobarbital Sodium</td>
			<td style="width:183px;">Sigma</td>
			<td style="width:119px;">P3761</td>
			<td>To anesthetize mice</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Normal saline</td>
			<td style="width:183px;">SACKLER</td>
			<td style="width:119px;">SR8572EP-15</td>
			<td>To prevent drying of mice eyes</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">75% Ethanol</td>
			<td style="width:183px;">Nanjing Reagent</td>
			<td style="width:119px;">C0691560275</td>
			<td>Disinfection</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Medical cotton ball</td>
			<td style="width:183px;">Shuitao</td>
			<td style="width:119px;">1278298933</td>
			<td>Disinfection</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Shaver</td>
			<td style="width:183px;">Kemei</td>
			<td style="width:119px;">KM-3018</td>
			<td>To shave the fur</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Scissor</td>
			<td style="width:183px;">RWD LIFE SCIENCE</td>
			<td style="width:119px;">S12005-10</td>
			<td>To incise skin</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Suture</td>
			<td style="width:183px;">RWD LIFE SCIENCE</td>
			<td style="width:119px;">F34001-01</td>
			<td>To suture skin</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Needle holder</td>
			<td style="width:183px;">RWD LIFE SCIENCE</td>
			<td style="width:119px;">F33001-01</td>
			<td>To suture skin</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Needle</td>
			<td style="width:183px;">RWD LIFE SCIENCE</td>
			<td style="width:119px;">R14003-12</td>
			<td>To suture skin</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Vessel forceps</td>
			<td style="width:183px;">RWD LIFE SCIENCE</td>
			<td style="width:119px;">F22003-09</td>
			<td>To suture skin</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Scalpel</td>
			<td style="width:183px;">RWD LIFE SCIENCE</td>
			<td style="width:119px;">S31010-01</td>
			<td>To harvest calvaria</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Tweezers</td>
			<td style="width:183px;">RWD LIFE SCIENCE</td>
			<td style="width:119px;">F12006-10</td>
			<td>To harvest calvaria</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">100 &#181;L pipettes</td>
			<td style="width:183px;">Eppendorf</td>
			<td style="width:119px;">3120000240</td>
			<td>To embed particles suspension in the calvatias</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">100 &#181;L pipette tips</td>
			<td style="width:183px;">AXYGEN</td>
			<td style="width:119px;">T-200-Y</td>
			<td>To embed particles suspension in the calvatias</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">5 ml Microtubes</td>
			<td style="width:183px;">Taizhou Weierkang Medical Supplies co., LTD</td>
			<td>W621</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">4% Paraformaldehyde</td>
			<td style="width:183px;">Servicebio</td>
			<td style="width:119px;">G1101</td>
			<td>Fixation</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Micro Computed Tomography&#160;</td>
			<td style="width:183px;">SkyScan</td>
			<td style="width:119px;">SkyScan1176</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Ethylene Diamine Tetraacetic Acid</td>
			<td style="width:183px;">Servicebio</td>
			<td style="width:119px;">G1105</td>
			<td>Decalcification</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Paraffin</td>
			<td style="width:183px;">Servicebio</td>
			<td style="width:119px;">#0001</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Paraffin slicing machine</td>
			<td style="width:183px;">Leica</td>
			<td style="width:119px;">RM2125RTS</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Glass slide</td>
			<td style="width:183px;">Servicebio</td>
			<td style="width:119px;">G6004</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Cover glass</td>
			<td style="width:183px;">Servicebio</td>
			<td style="width:119px;">200</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">HE staining kit</td>
			<td style="width:183px;">Servicebio</td>
			<td style="width:119px;">#1-5</td>
			<td>HE staining</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Light microscope</td>
			<td style="width:183px;">Nikon</td>
			<td style="width:119px;">E200</td>
			<td></td>
		</tr>
	</tbody>
</table>

construction and evaluation of a murine calvarial osteolysis model by exposure to cocrmo particles in aseptic loosening

Wear particle-induced osteolysis is a major cause of aseptic loosening in arthroplasty failure, but the underlying mechanism remains unclear. Due to long follow-ups necessary for detection and sporadic occurrence, it is challenging to assess the pathogenesis ofparticle-induced osteolysis in clinical cases. Hence, optimal animal models are required for further studies. The murine model of calvarial osteolysis established by exposure to CoCrMo particles is an effective and valid tool for assessing the interactions between particles and various cells in aseptic loosening. In this model, CoCrMo particles were first obtained by high-vacuum three-electrode direct current and resuspended in phosphate-buffered saline at a concentration of 50 mg/mL. Then, 50 &#181;L of the resulting suspension was applied to the middle of the murine calvaria after separation of the cranial periosteum by sharp dissection. After two weeks, the mice were sacrificed, and calvaria specimens were harvested; qualitative and quantitative evaluations were performed by hematoxylin and eosin staining and micro computed tomography. The strengths of this model include procedure simplicity, quantitative evaluation of bone loss, rapidity of osteolysis development, potential use transgenic or knockout models, and a relatively low cost. However, this model cannot to be used to assess the mechanical force and chronic effects of particles in aseptic loosening. Murine calvarial osteolysis model generated by exposure to CoCrMo particles is an ideal tool for assessing the interactions between wear particles and various cells, e.g., macrophages, fibroblasts, osteoblasts and osteoclasts, in aseptic loosening.

The in-house produced nanoscale CoCrMo particles were around 50 nm (standard error of 3.56) in diameter, as quantified by TEM (Figure 2). After exposure of mouse calvarias to CoCrMo particles, the animals (n=6 per group) were maintained for another two weeks. Within the two weeks, the calvarial incision was completely healed, and the suture may fall. Any local infection or nonunion may affect bone loss assessment. After mouse sacrifice, calvaria samples were ...

Watch this Scientific Journal Video about Construction and Evaluation of a Murine Calvarial Osteolysis Model by Exposure to CoCrMo Particles in Aseptic Loosening at JoVE.com

Construction and Evaluation of a Murine Calvarial Osteolysis Model by Exposure to CoCrMo Particles in Aseptic Loosening

This manuscript describes a murine calvarial osteolysis model by exposure to CoCrMo particles, which constitutes an ideal animal model for assessing the interactions between wear particles and various cells in aseptic loosening.

Wear particle-induced osteolysis is a major cause of aseptic loosening in arthroplasty failure, but the underlying mechanism remains unclear. Due to long ...

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