December 29th, 2017
•Here, we describe a protocol for chromatin immunoprecipitation of modified histones from the budding yeast Saccharomyces cerevisiae. Immunoprecipitated DNA is subsequently used for quantitative PCR to interrogate the abundance and localization of histone post-translational modifications throughout the genome.
Related Videos
Analysis of the c-KIT Ligand Promoter Using Chromatin Immunoprecipitation
Genome-wide Mapping of Protein-DNA Interactions with ChEC-seq in Saccharomyces cerevisiae
Chromatin Immunoprecipitation (ChIP) in Mouse T-cell Lines
Generation of Native Chromatin Immunoprecipitation Sequencing Libraries for Nucleosome Density Analysis
Chromatin Immunoprecipitation (ChIP) Protocol for Low-abundance Embryonic Samples
Chromatin Immunoprecipitation of Murine Brown Adipose Tissue
Chromatin Immunoprecipitation Assay Using Micrococcal Nucleases in Mammalian Cells
Discovering CsgD Regulatory Targets in Salmonella Biofilm Using Chromatin Immunoprecipitation and High-Throughput Sequencing (ChIP-seq)
High-Resolution Mapping of Protein-DNA Interactions in Mouse Stem Cell-Derived Neurons using Chromatin Immunoprecipitation-Exonuclease (ChIP-Exo)
Genome-wide Analysis of Histone Modifications Distribution using the Chromatin Immunoprecipitation Sequencing Method in Magnaporthe oryzae
Copyright © 2024 MyJoVE Corporation. 판권 소유