Identification of Neuromuscular Junction using Fluorescence
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Results: Current-clamp and Voltage-clamp Recording of Membrane Properties
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Conclusion
필기록
The overall goal of this procedure is to isolate the levator auris longus muscle and its nerve in order to record synaptic currents at the neural muscular junction. This method can help answer key questions in synaptic physiology such as the end p
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The protocol described in this paper uses the mouse levator auris longus (LAL) muscle to record spontaneous and nerve-evoked postsynaptic potentials (current-clamp) and currents (voltage-clamp) at the neuromuscular junction. Use of this technique can provide key insights into mechanisms of synaptic transmission under normal and disease conditions.