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Time-Resolved Fluorescence Imaging and Analysis of Cancer Cell Invasion in the 3D Spheroid Model

6.1K Views

07:42 min

January 30th, 2021

January 30th, 2021

6,149 Views

0:05

Introduction

0:39

Spheroid Imaging Device (SID) Fabrication

4:51

Spheroid Collagen Embedding

6:06

Results: Representative Spheroid Imaging

7:06

Conclusion

Transcribir

This protocol can be used to study the mechanisms governing the invasion of cancer cells into the extracellular matrix in real-time. The main advantage of this technique is that it uses a simple spheroid imaging device, which makes the spheroid in

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Presented here is a protocol for the fabrication of a spheroid imaging device. This device enables dynamic or longitudinal fluorescence imaging of cancer cell spheroids. The protocol also offers a simple image processing procedure for the analysis of cancer cell invasion.

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