Perform Layer‐by‐Layer Deposition of a Prime Layer, DNA Plasmids, and Silk Layers
4:44
Dissolution of SiO2Cores and Imaging of Silk Fibroin Microcapsules
5:42
Estimation of the Permeability of Hollow Microcapsules Using Molecular Weight Cut‐Off (MWCO) Method
6:42
In Vitro Activation of Synthetic Theophylline Riboswitch in Silk Microcapsules
7:28
Results: Microscopy Studies, Membrane Permeability, and Gene Expression Kinetics of Silk Fibroin Microcapsules
9:25
Conclusion
필기록
The significance of this protocol is that it provides a means for keeping DNA-encoded sensors functional by immobilizing DNA plasmid templates into semipermeable biocompatible microcapsules. The proposed DNA immobilization technique is versatile.
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The protocol describes the formation of robust and biocompatible DNA-laden microcapsules as multiplexed in vitro biosensors capable of tracking several ligands.