Tissue Processing and Generation of Cartilage Discs
1:14
Cartilage Disc Staining and Sorting
2:43
Biomechanical Approach of Cartilage Explants
6:11
Results: AFM Measurements of Cartilage Discs Based on Cellular Pattern Organization
7:25
Conclusion
필기록
The goal of this protocol was to create an in vitro model for studying articular cartilage mechanical properties during the onset and the progression of osteoarthritis. The main advantage of the present technique is that the cartilage explants are
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We present a step-by-step approach to identify and address the most common problems associated with atomic force microscopy micro-indentations. We exemplify the emerging problems on native human articular cartilage explants characterized by various degrees of osteoarthritis-driven degeneration.