Floral Staging of Septum Donors, Stigma Donors, and Pollen Donors
2:04
Septum Dissection
3:20
Stigma Dissection
3:55
Imaging
4:46
Results: Live Imaging of Arabidopsis Pollen Tube Reception and Double Fertilization
6:02
Conclusion
필기록
This method will facilitate research on sexual reproduction in Arabidopsis by allowing for the observation of gametophyte interactions with high efficiency and with high sample size number per imaging session. The main advantage of this technique
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Here, we describe an improvement of the semi-in vitro (SIV) method for observing pollen tube guidance and reception in Arabidopsis thaliana, which increases the receptivity of ovules. The high-throughput SIV cum septum method may be coupled with gametophyte marker lines and genetically encoded biosensors to monitor the dynamic process of fertilization.