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Generation of Greater Bacterial Biofilm Biomass using PCR-Plate Deep Well Microplate Devices

7.3K Views

10:57 min

April 22nd, 2022

DOI :

10.3791/63069-v

April 22nd, 2022


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Bacterial Biofilm

Hoofdstukken in deze video

0:15

Introduction

1:41

Days 0-2: Aseptic Culture Preparation for Biofilm Group

2:05

Day 2: Preparation and Inoculation of Deep Well Biofilm Plates

3:08

Day 3, Part 1: Biofilm Biomass Determination from Device Using CV Staining

4:10

Day 3, Part 2: Challenging Biofilms with Antimicrobial to Calculate Their 24 Hour Antimicrobial MBEC Value

4:54

Day 4: Recovery of Biofilm Biomass from Pegged Lids and Day 5: Determination of Device MBEC Values

5:44

Representative Results

9:07

Conclusion

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