Determining the Role of Maternally-Expressed Genes in Early Development with Maternal Crispants

0:56

Synthesis of Guide RNAs

2:03

Microinjection of CRISPR-Cas9 Cocktail into a One-Cell Zebrafish Embryo to Generate Maternal Crispants

3:07

Screening for Somatic INDELs in F0 Injected Embryos

4:54

Identification of Maternal-Effect Phenotypes in Maternal Crispant Embryos

6:21

Sequencing Alleles in Maternal Crispant Haploids

8:18

Results: Identification of Maternal Effect Phenotypes in a Rapid and Resource Efficient Manner

9:08

Conclusion