Determining the Role of Maternally-Expressed Genes in Early Development with Maternal Crispants
2.2K Views
•
10:08 min
•
December 21st, 2021
DOI :
December 21st, 2021
•Chapters in this video
0:05
Introduction
0:56
Synthesis of Guide RNAs
2:03
Microinjection of CRISPR-Cas9 Cocktail into a One-Cell Zebrafish Embryo to Generate Maternal Crispants
3:07
Screening for Somatic INDELs in F0 Injected Embryos
4:54
Identification of Maternal-Effect Phenotypes in Maternal Crispant Embryos
6:21
Sequencing Alleles in Maternal Crispant Haploids
8:18
Results: Identification of Maternal Effect Phenotypes in a Rapid and Resource Efficient Manner
9:08
Conclusion
Related Videos
09:22
Manipulation and In Vitro Maturation of Xenopus laevis Oocytes, Followed by Intracytoplasmic Sperm Injection, to Study Embryonic Development
22.5K Views
11:25
Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos
10.8K Views
10:39
Functional Manipulation of Maternal Gene Products Using In Vitro Oocyte Maturation in Zebrafish
11.3K Views
09:39
CRISPR-mediated Loss of Function Analysis in Cerebellar Granule Cells Using In Utero Electroporation-based Gene Transfer
9.6K Views
07:39
Mouse In Vivo Placental Targeted CRISPR Manipulation
2.5K Views
07:13
Induction of Maternal Immune Activation in Mice at Mid-gestation Stage with Viral Mimic Poly(I:C)
18.3K Views
08:37
Preparation of Small RNA Libraries for Sequencing from Early Mouse Embryos
5.3K Views
14:08
Blastomere Explants to Test for Cell Fate Commitment During Embryonic Development
15.2K Views
08:36
Separation of Mouse Embryonic Facial Ectoderm and Mesenchyme
10.9K Views
08:05
Efficient and Rapid Isolation of Early-stage Embryos from Arabidopsis thaliana Seeds
17.7K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved