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W tym Artykule

  • Podsumowanie
  • Streszczenie
  • Wprowadzenie
  • Protokół
  • Wyniki
  • Dyskusje
  • Ujawnienia
  • Podziękowania
  • Materiały
  • Odniesienia
  • Przedruki i uprawnienia

Podsumowanie

Here, we discuss a series of protocols for induction and validation of cellular senescence in cultured cells. We focus on different senescence-inducing stimuli and describe the quantification of common senescence-associated markers. We provide technical details using fibroblasts as a model, but the protocols can be adapted to various cellular models.

Streszczenie

Cellular senescence is a state of permanent cell cycle arrest activated in response to different damaging stimuli. Activation of cellular senescence is a hallmark of various pathophysiological conditions including tumor suppression, tissue remodeling and aging. The inducers of cellular senescence in vivo are still poorly characterized. However, a number of stimuli can be used to promote cellular senescence ex vivo. Among them, most common senescence-inducers are replicative exhaustion, ionizing and non-ionizing radiation, genotoxic drugs, oxidative stress, and demethylating and acetylating agents. Here, we will provide detailed instructions on how to use these stimuli to induce fibroblasts into senescence. This protocol can easily be adapted for different types of primary cells and cell lines, including cancer cells. We also describe different methods for the validation of senescence induction. In particular, we focus on measuring the activity of the lysosomal enzyme Senescence-Associated β-galactosidase (SA-β-gal), the rate of DNA synthesis using 5-ethynyl-2'-deoxyuridine (EdU) incorporation assay, the levels of expression of the cell cycle inhibitors p16 and p21, and the expression and secretion of members of the Senescence-Associated Secretory Phenotype (SASP). Finally, we provide example results and discuss further applications of these protocols.

Wprowadzenie

In 1961, Hayflick and Moorhead reported that primary fibroblasts in culture lose their proliferative potential after successive passages1. This process is caused by the sequential shortening of telomeres after each cell division. When telomeres reach a critically short length, they are recognized by the DNA-damage response (DDR) that activates an irreversible arrest of proliferation — also defined as replicative senescence. Replicative senescence is currently one of the many stimuli that are known to induce a state of permanent cell cycle arrest that renders cells insensitive both to mitogens and to apoptotic signals2,3. The senescence program is normally characterized by additional features including high lysosomal activity, mitochondrial dysfunction, nuclear changes, chromatin rearrangements, endoplasmic reticulum stress, DNA damage and a senescence-associated secretory phenotype (SASP)3,4. Senescent cells have multiple functions in the body: development, wound healing and tumor suppression2. Equally, they are known to play an important role in aging and, paradoxically, in tumor progression5. The negative, and partially contradictory, effects of senescence are often attributed to the SASP6.

Recently, it was shown that elimination of senescent cells from mice leads to lifespan extension and to elimination of many of the aging features7,8,9,10,11,12. In the same way, multiple drugs have been developed to either eliminate senescent cells (senolytics) or to target the SASP13,14. The anti-aging therapeutic potential has recently attracted more attention to the field.

The study of mechanisms associated to cellular senescence and the screenings for pharmacological interventions heavily rely on ex vivo models, particularly on human primary fibroblasts. While there are some common features activated by diverse senescence inducers, a large variability in the senescence phenotype is observed and dependent on various factors including cell type, stimulus and time point3,15,16,17. It is imperative to consider the heterogeneity for studying and targeting senescent cells. Therefore, this protocol aims to provide a series of methods used to induce senescence in primary fibroblasts by using different treatments. As it will be explained, the methods can easily be adapted to other cell types.

Apart from replicative senescence, we describe five other senescence-inducing treatments: ionizing radiation, ultraviolet (UV) radiation, doxorubicin, oxidative stress and epigenetic changes (namely promotion of histone acetylation or DNA demethylation). Both, ionizing radiation and UV-radiation cause direct DNA damage and, at the appropriate dose, trigger senescence18,19. Doxorubicin also causes senescence mainly through DNA damage by intercalating into the DNA and disrupting topoisomerase II function and thus halting DNA repair mechanisms20. The expression of genes essential for senescence is normally controlled by histone acetylation and DNA methylation. As a consequence, histone deacetylase inhibitors (e.g., sodium butyrate and SAHA) and DNA demethylating (e.g., 5-aza) agents trigger senescence in otherwise normal cells21,22.

Finally, four of the most common markers associated to senescent cells will be explained: activity of the senescence associated-β-galactosidase (SA-β-gal), rate of DNA synthesis by EdU incorporation assay, overexpression of the cell cycle regulators and cyclin-dependent kinase inhibitors p16 and p21, and overexpression and secretion of members of the SASP.

Protokół

1. General Preparation

  1. Prepare D10 medium. Supplement DMEM medium-Glutamax with 10% FBS and 1% penicillin/streptomycin (Final concentration: 100 U/mL).
  2. Prepare sterile PBS. Dissolve the tablets in water according to manufacturer’s instructions. Sterilize by autoclave.
  3. Prepare 1x trypsin. Dilute 5 mL of Trypsin-Versene EDTA/10x 1:10 in 45 mL of sterile PBS.
    Note: Throughout the protocol, we use cell culture conditions that are closer to the physiological conditions for primary fibroblasts. This means that we incubate cells at 37 °C and 5% CO2 as is normally done but using 5% O2 instead of the “standard” 20% O2.
  4. Handle all samples in sterile conditions by using a laminar flow hood, lab coat and gloves. Keep cells at 20% O2 (room conditions) only while being handled.

2. Induction of Senescence

  1. Replicative Senescence
    1. While handling cells or any material that will be in contact with them (pipets, flasks, media, etc.) work under sterile conditions, by using a laminar flow hood, lab coat and gloves. Keep cells at 20% O2 (room conditions) only while being handled.
    2. Seed 7 x 105 viable primary fibroblasts in a low population doubling (PD) in a T75 flask (~9.3 x 103 cells/cm2) containing 10 mL of D10.
    3. Grow the cells in a cell incubator at 37 °C with 5% CO2 and 5% O2 until they reach 70–80% confluence (3–4 days for proliferating cultures in a low PD).
    4. Detach the cells using 3 mL of 1x trypsin and incubating for ~5–7 min in a cell culture incubator at 37 °C with 5% CO2 and 5% O2. Monitor the cells regularly with a cell culture microscope to check the detaching process.
    5. When cells are spherical, stop the trypsin reaction by adding 9 mL of D10. Do not incubate longer than 10 min.
    6. Spin the cells at 300 x g for 5 min. Cells will form a pellet at the bottom of the tube, while debris and smaller particles will remain in the supernatant.
    7. Remove the supernatant and dissolve the cell pellet in 1 mL of D10 and perform a cell count using an automated cell counter according to manufacturer’s instructions or a Neubauer chamber for manual counting. While counting, include an assay to check the viability of the cells (e.g., Trypan Blue exclusion23).
    8. Calculate the cumulative PD using this formula:
      PDnew = 3.32*(LOG(cell number total)-(LOG(cell number seeded)) + PDold
      Cell number total = all cells counted: dead and alive.
      Cell number seeded = number of viable cells seeded (8x105 cells).
      PDold = population doubling at the moment of seeding.
      PDnew = population doubling at the moment of counting (after incubation).
      NOTE: If 7 x 105 primary fibroblasts (PD 35.2) were seeded on a T-75 flask and, after 4 days they reach 80% confluence and are split again, counting now 1.3 x 106 total cells (dead+alive).
      PDnew = 3.32*(LOG(1,300,000 cells)-LOG(700,000))+35.2
      PDnew = 36.1
    9. Reseed 8 x 105 cells in a new T75, repeating steps 2.1.2–2.1.8.
      NOTE: After multiple consecutive passages, the culture will take longer to get confluent until cells stop dividing at all. Once cells stop dividing, test for senescence markers and/or harvest for downstream applications.
    10. Consider that the bigger size of senescent cells may cause the culture to appear full and yet have a low cell count. Thus, senescence can be assumed when the PD is stable and other senescence markers appear in the culture (see protocols 3.1–3.5).
      Note: Use proliferating primary fibroblasts as control.
  2. Ionizing Radiation-induced Senescence
    1. While handling cells or any material that will be in contact with them (pipets, flasks, media, etc.), work under sterile conditions by using a laminar flow hood, lab coat and gloves. Keep cells at 20% O2 (room conditions) only while being handled.
    2. Seed 7 x 105 viable primary fibroblasts in a low PD in a T75 flask (~9.3 x 103 cells/cm2) containing 10 mL of D10.
    3. Incubate the cells overnight in a cell culture incubator at 37 °C with 5% CO2 and 5% O2.
    4. Expose the cells to 10 gray of gamma irradiation according to the instructions of the machine in use.
    5. Aspirate the medium from the cells and replace with 10 mL of D10.
    6. Incubate the cells in 10 mL of D10 in a cell culture incubator at 37 °C with 5% CO2 and 5% O2 for another 10 days replacing the medium regularly, approximately every 3 days.
    7. After 10 days, test for senescence markers and/or use the cells for downstream applications.
      NOTE: Use proliferating primary fibroblasts of the same PD (before irradiation) as control.
  3. Ultraviolet (UV) radiation-Induced Senescence
    1. While handling cells or any material that will be in contact with them (pipets, flasks, media, etc.), work under sterile conditions by using a laminar flow hood, lab coat and gloves. Keep cells at 20% O2 (room conditions) only while being handled.
    2. Seed 1.5–2 x 105 viable primary fibroblasts in a low PD in one well of a 6-well plate (1.5–2.0 x 104 cells/cm2), add 2 mL of D10 medium.
    3. Place the cells in a cell culture incubator at 37 °C with 5% CO2 and 5% O2 and allow them to adhere to the plastic for at least 5 h.
    4. Take off the medium from the cells. Place the 6-well plate in the middle of the UV-radiation chamber and take the plastic lid off. Irradiate with UVB, 20–30 mJ/cm2.
    5. Add 2 mL of medium per well.
    6. Incubate the cells in 2 mL of D10 in a cell culture incubator at 37 °C with 5% CO2 and 5% O2 for another 7 days replacing the medium regularly, approximately every 3 days.
      NOTE: After 7 days, cells can be tested for senescence markers and used for downstream applications. Use proliferating primary fibroblasts of the same PD (before irradiation) as control.
  4. Doxorubicin-induced Senescence
    1. Prepare 1,000x Doxorubicin stock solution: Make a 250 µM stock of Doxorubicin in 1x PBS, filter-sterilize the solution and aliquot in 500 µL per sterile tube. Store the doxorubicin stock at -80 °C.
    2. While handling cells or any material that will be in contact with them (pipets, flasks, media, etc.), work under sterile conditions by using a laminar flow hood, lab coat and gloves. Keep cells at 20% O2 (room conditions) only while being handled.
    3. Seed 7 x 105 viable primary fibroblasts in a low PD in a T75 flask (~9.3 x 103 cells/cm2) containing 10 mL of D10.
    4. Incubate the cells overnight in a cell culture incubator at 37 °C with 5% CO2 and 5% O2.
    5. Dilute 11 µL of the 1,000x doxorubicin stock solution in 11 mL of D10 to a final concentration of 250 nM.
    6. Aspirate the medium from the cells and replace with 10 mL of D10 + doxorubicin.
    7. Incubate the cells for exactly 24 h in a cell culture incubator at 37 °C with 5% CO2 and 5% O2.
    8. Aspirate the medium from the cells and carefully wash once with 10 mL of D10.
    9. Incubate the cells in 10 mL of D10 for another 6 days replacing the medium regularly, approximately every 3 days.
    10. At day 7, test for senescence markers and/or use for downstream applications.
      NOTE: As control, use proliferating primary fibroblasts of the same PD treated for 24 h with vehicle (PBS) 1:1,000 in D10.
  5. Oxidative Stress-induced Senescence
    1. While handling cells or any material that will be in contact with them (pipets, flasks, media, etc.) work under sterile conditions by using a laminar flow hood, lab coat and gloves. Keep cells at 20% O2 (room conditions) only while being handled.
    2. Seed 7 x 105 viable primary fibroblasts in a low PD in a T75 flask (~9.3 x 103 cells/cm2) containing 10 mL of D10.
    3. Incubate the cells overnight in a cell incubator at 37 °C with 5% CO2 and 5% O2.
    4. Prepare a solution of ~200 μM hydrogen peroxide in D10 medium by adding 22.6 μL of 30% hydrogen peroxide in 11 mL of D10.
      NOTE: Optimize the treatment for the cell type of interest by making a curve dose-response to evaluate toxicity.
    5. Aspirate the medium from the cells and add 10 mL of the freshly prepared D10 medium+hydrogen peroxide. Incubate for 2 h at 37 °C with 5% CO2 and 5% O2.
    6. Aspirate the medium from the cells and wash once with fresh D10 without hydrogen peroxide.
    7. Add 10 mL of D10 without hydrogen peroxide.
    8. Incubate for 48 h in a cell culture incubator at 37 °C with 5% CO2 and 5% O2.
    9. Repeat steps 2.5.4–2.5.8 two times more for a total of three treatments.
    10. Check for senescence markers or harvest for downstream applications.
      Note: As control, use proliferating cells of the same PD treated with 22.6 µL of sterile water in D10 for 2 h.
  6. Epigenetically-induced Senescence
    1. Prepare stock and working solutions for the small molecule(s) to use according to Table 1. Filter-sterilize the solutions. Aliquot in sterile tubes. Store at -20 °C.
    2. While handling cells or any material that will be in contact with them (pipets, flasks, media, etc.) work under sterile conditions, for instance, by using a laminar flow hood, lab coat and gloves. Keep cells at 20% O2 (room conditions) only while being handled.
    3. Seed 7 x 105 viable primary fibroblasts in a low PD in a T75 flask (~9.3 x 103 cells/cm2) containing 10 mL of D10.
    4. Incubate the cells overnight in a cell culture incubator at 37 °C with 5% CO2 and 5% O2.
    5. Prepare 11 mL of D10 medium + working solution for the desired treatment. The exact dilution per treatment can be seen in Table 1.
      1. Prepare 11 µL of 1 mM SAHA working solution in 11 mL of D10.
      2. Prepare 44 µL of 1 M sodium butyrate working solution in 11 mL of D10.
      3. Prepare 11 µL of 10 mM 5-aza working solution in 11 mL of D10.
        Note: Optimize the treatments for the cell type of interest by, for instance, making a curve dose-response to evaluate toxicity.
    6. Add D10 medium + working solution for the desired treatment to the culture.
    7. Incubate for 24 h in a cell culture incubator at 37 °C with 5% CO2 and 5% O2.
    8. Repeat steps 2.6.4–2.6.6 two times more, for a total of three treatments.
    9. Change medium for simple D10 without drug.
    10. After 3 days more, cells become senescent and ready for testing of senescence markers and downstream applications.
      NOTE: As control, use proliferating primary fibroblasts of the same PD treated for three days with D10 medium+vehicle. The D10 medium+vehicle needs to be refreshed every 24 h during those three days. The vehicle depends on the treatment used: 1:1,000 DMSO for SAHA and 5-aza and 1:250 sterile water for Sodium Butyrate.

3. Markers of Senescence

  1. Preparation
    1. Prepare 20 mg/mL X-gal: Dissolve 20 mg of X-gal in 1 mL of dimethylformamide or DMSO. Store at -20 °C protected from light.
    2. Prepare 0.1 M citric acid solution: Dissolve 2.1 g of citric acid monohydrate in 100 mL of water. Store at room temperature.
    3. Prepare 0.2 M sodium phosphate solution: Dissolve 2.84 g of sodium dibasic phosphate or 3.56 g of sodium dibasic phosphate dehydrate in 100 mL of water. Store at room temperature.
    4. Prepare 0.2 M citric acid/sodium phosphate pH 6.0: Dissolve 36.85 mL of 0.1 M citric acid solution and 63.15 mL of 0.2 M sodium phosphate. Adjust exactly to pH 6.0. Store at room temperature.
    5. Prepare 100 mM potassium ferrocyanide: Dissolve 2.1 g of potassium ferrocyanide in 50 mL of water. Store at 4 °C protected from light.
    6. Prepare 100 mM potassium ferricyanide: Dissolve 1.7 g of potassium ferricyanide in 50 mL of water. Store at 4 °C protected from light.
    7. Prepare 5 M sodium chloride: Dissolve 14.6 g of sodium chloride in 50 mL of water. Store at room temperature.
    8. Prepare 1 M magnesium chloride: Dissolve 4.8 g of magnesium chloride in 50 mL of water. Store at room temperature.
    9. Prepare 2% formaldehyde + 0.2% glutaraldehyde in PBS: Dissolve 800 µL of 25% glutaraldehyde and 12.5 µL of 16% formaldehyde in 100 μL of PBS. Store at room temperature protected from light.
    10. Prepare staining solution fresh according to Table 2.
  2. Senescence-associated β-galactosidase Staining
    1. For each sample, seed 1 x 104 cells in at least one well of a 24-well plate (5.2 x 103 cells/cm2) containing 500 µL of D10 so that the cells are sparse. Treatments (if applicable) can be performed directly on this plate or, alternatively, cells treated already can be re-seeded into a 24-well plate.
    2. Incubate the cells overnight at 37 °C with 5% CO2 and 5% O2.
    3. Wash cells two times with 500 μL of PBS.
    4. Fix 3–5 min at room temperature using 500 μL/well of 2% formaldehyde + 0.2% glutaraldehyde in PBS.
    5. Wash cells two times with 500 μL of PBS.
    6. Prepare fresh staining solution, according to the number of samples to stain.
    7. Add staining solution (500 µL/well) and seal the plate with parafilm to avoid evaporation.
      NOTE: Evaporation may cause crystals to form and hinder the observation under the microscope.
    8. Incubate cells in the dark (e.g. covered in aluminum foil) at 37 °C in a dry incubator (without CO2) for 12–16 h.
      Note: CO2 may affect the pH and therefore modify the results. Some cell types may require shorter incubation times.
    9. Wash two times with 500 μL of PBS.
    10. Assess the results. Positive cells present a blue (mostly) perinuclear staining under a normal light microscope (Figure 1A).
    11. For quantification, observe at least 100 cells per sample and count the number of positive cells. Since senescent cells change shape, it is often difficult to define the cell boundaries and to count the cells. Counterstain with DAPI to facilitate visualization and quantification of individual cells (Figure 1B). Quantify the samples (percentage of positive cells versus total amount of cells) using a fluorescent microscope (Figure 1C). Take multiple pictures of the same sample so that at the end you can count at least 100 single-cells and evaluate the percentage of SA-β-gal positive cells in them.
    12. Compare results of senescent cells versus their appropriate control for the treatment used.
      NOTE: A co-staining with EdU on the same sample is possible. Consider that cells should then be seeded on coverslips.
  3. EdU Incorporation Assay
    1. Put a coverslip/well in a 24-well plate according to the number of samples to assess.
    2. Seed 1 x 104 cells in at least one well of a 24-well plate (5.2 x 103 cells/cm2) per condition containing 500 µL of D10 so that the cells are sparse. Treatments (if applicable) can be performed directly on this plate or, alternatively, already treated cells can be re-seeded into a 24-well plate.
    3. Incubate the cells overnight at 37 °C with 5% CO2 and 5% O2.
    4. Make a 20 µM solution of EdU in D10 (1:250) according to the number of samples to treat (250 μL per sample).
    5. Remove half of the medium (250 μL) from each well to treat and replace it with the D10 + EdU solution that was just prepared. Final EdU concentration is 10 µM.
    6. Incubate 18–24 h in a cell culture incubator at 37 °C with 5% CO2 and 5% O2. Use the same incubation time in control and senescent cells.
    7. Wash 2x with 500 μL of PBS.
    8. Fix the cells for 10 min with 500 μL of 4% formaldehyde in PBS.
    9. Incubate 5 min in 500 μL of 100 mM Tris (pH 7.6).
    10. Permeabilize the cells for 10 min in 500 μL of 0.1% Triton X-100 in PBS.
    11. Wash 3x with PBS.
    12. Prepare 50 μL of label mix for each coverslip, adding the components in the following order: 44.45 µL of PBS, 0.5 µL of Cu(II)SO4, 0.05 µL of sulfo-Cy3-azide, 5 µL of sodium-ascorbate.
    13. Put 50 μL of the label mix on a piece of parafilm. Lift the coverslip with the aid of a pair of tweezers and a needle and let it rest on top of the label mix, with the surface containing the cells facing downwards. Ensure that there are no bubbles and that the whole surface of the coverslip is touching the label mix. Incubate for 30 min in the dark.
    14. Put the cells back in the wells of the 24-well plate and wash them three times with PBS.
    15. Mount with mounting media (including DAPI to visualize nuclei) onto glass slides and let them dry overnight.
    16. Visualize the EdU incorporation using a fluorescent microscope. Use a filter appropriate for Cy3 (excitation/emission: 552/570 nm).
    17. Take multiple pictures of the cells for later quantification of at least 100 cells per condition (Cy3 and DAPI).
    18. Quantify the percentage of cells that incorporated EdU by using the following formula:
      EdU positive cells (%) = (EdU positive cell count (Cy3)/total cell count (DAPI)) * 100
    19. Compare results of senescent cells versus their appropriate control for the treatment used.
      Note: A co-staining with SA-β-gal on the same sample is possible. Consider that cells should still be seeded on coverslips.
  4. Gene Expression of p16, p21 and SASP
    1. Prepare primer-sets of Genes of Interest (50 µM each primer).
      NOTE: A qPCR of p16, p21 and some relevant SASP factors is informative of the senescence status. The protocol described here makes use of the Universal Probe Library (UPL) system for relative quantification using a real time-PCR. Table 3 shows an overview of the primers used for detection of the CDK inhibitors p16 and p21 and of the most relevant SASP members, as well as for Tubulin and Actin, which serve as reference genes for the assay. The last column of Table 3 enlists the particular UPL probe to be used for each assay.
    2. Prepare separate qPCR reaction mix for the desired assays. Always include the reference gene as well according to Table 4.
    3. Run all samples in duplicate or triplicate.
    4. Load 7.5 µL/well of the qPCR reaction mix on a 384-well plate.
    5. Add ~5 ng of cDNA dissolved in 2.5 µL of RNase-free water.
      NOTE: It is preferable to have similar amounts of cDNA for all the samples to be compared. This can be achieved by using equal amounts of RNA for the reverse transcription.
    6. Cover the plate with a seal and make sure that it sticks correctly covering evenly all the wells on the plate.
    7. Spin the plate at 2,000 x g for 1 min.
    8. Run the plate in the Lightcycler for 40 cycles using the following protocol:
      95 °C for 7 min
      40 cycles of 95 °C for 5 s and 60 °C for 30 s
      37 °C for 1 min
    9. For the analysis of the results, use the method proposed for Livak and colleagues to analyze qPCR data24. Use either Tubulin or Actin as reference genes to calculate the ΔCt value and use the appropriate control to calculate the for the ΔΔCt value for the specific senescence-inducing treatment.
  5. Protein expression and secretion of IL6
    1. Seed 5–10 x 104 cells in at least one well of a 6-well plate (5.2–10.5 x 103 cells/cm2) per condition containing 2 mL of D10. Treatments (if applicable) can be performed directly on this plate or, alternatively, cells treated already can be re-seeded into a 24-well plate. Let it stand overnight after seeding.
    2. Remove the medium and replace for 2 mL of DMEM medium without FBS. Incubate in normal conditions for 24 h.
    3. Collect the medium in a 15 mL tube.
    4. Centrifuge the sample at 300 x g for 5 min. Medium can be stored at -80 °C until processed.
    5. Follow manufacturer’s instructions to perform the ELISA.
    6. Compare results of senescent cells versus the appropriate control for the specific senescence-inducing treatment

Wyniki

Enrichment of SA-β-gal staining in senescent fibroblasts

Β-galactosidase (β-gal) is a lysosomal enzyme that is expressed in all cells and that has an optimum pH of 4.025,26. However, during senescence, lysosomes increase in size and, consequently, senescent cells accumulate β-gal. The increased amounts of this enzyme make it possible to detect its activi...

Dyskusje

The protocols explained here were optimized for human primary fibroblasts, particularly BJ and WI-38 cells. The protocols for replicative senescence, ionizing radiation and doxorubicin, have been successfully applied to other types of fibroblasts (HCA2 and IMR90) and in other cell types (namely neonatal melanocytes and keratinocytes or iPSC-derived cardiomyocytes) in our laboratory. However, adaptations for additional cell types can be optimized by adjusting some details such as the number of seeded cells, the methods an...

Ujawnienia

N/A

Podziękowania

We thank members of the Demaria lab for fruitful discussions, and Thijmen van Vliet for sharing data and protocol on the UV-induced senescence.

Materiały

NameCompanyCatalog NumberComments
DMEM Media - GlutaMAXGibco31966-047
Fetal Bovine SerumHycloneSV30160.03
Penicillin-Streptomycin (P/S; 10,000 U/ml)LonzaDE17-602E
Dimethyl Sulfoxide (DMSO)Sigma-AldrichSC-202581
Nuclease-Free Water (not DEPC-Treated)AmbionAM9937
T75 flaskSarstedt833911002
Trypsin/EDTA SolutionLonzaCC-5012
PBS tabletsGibco18912-014
1.5 ml microcentrifuge tubesSigma-AldrichT9661-1000EA
Corning 15 mL centrifuge tubesSigma-AldrichCLS430791
6-well plateSarstedt83.3920
24-well plateSarstedt83.3922
13mm round coverslipsSarstedt83.1840.002
SteriflipMerck ChemicalsSCGP00525
Cesium137-sourceIBL 637 Cesium-137γ-ray machine
UV radiation chamberOpsytec, Dr. Göbel BS-02
Doxorubicin dihydrochloride BioAustralis Fine ChemicalsBIA-D1202-1
Hydrogen peroxide solutionSigma-Aldrich7722-84-1
5-aza-2’-deoxycytidineSigma-AldrichA3656
SAHASigma-AldrichSML0061
Sodium Butyrate Sigma-AldrichB5887
X-gal (5-Bromo-4-chloro-3-indolyl-β-D-galactopyranoside)Fisher Scientific7240-90-6
Citric acid monohydrateSigma-Aldrich5949-29-1
Sodium dibasic phosphateAcros organics7782-85-6
Potassium ferrocyanide Fisher Scientific14459-95-1
Potassium ferricyanideFisher Scientific13746-66-2
Sodium ChlorideMerck Millipore7647-14-5
Magnesium ChlorideFisher Chemicals7791-18-6
25% glutaraldehydeFisher Scientific111-30-8,7732-18-5
16% formaldehyde (w/v)Thermo-Fisher Scientific28908
EdU (5-ethynyl-2’-deoxyuridine)Lumiprobe10540
Sulfo-Cyanine3 azide (Sulfo-Cy3-Azide)LumiprobeD1330
Sodium ascorbateSigma-AldrichA4034
Copper(II) sulfate pentahydrate (Cu(II)SO4.5H2O)Sigma-Aldrich209198
Triton X-100Acros organics215682500
TRIS baseRoche11814273001
LightCycler 480 Multiwell Plate 384, white Roche4729749001
Lightcycler 480 sealing foil Roche4729757001
Sensifast Probe Lo-ROX kit BiolineBIO-84020
UPL Probe LibrarySigma-AldrichVarious
Human IL-6 DuoSet ELISAR&DD6050
Bio-Rad TC20Bio-Rad
Counting slidesBio-Rad145-0017
Dry incubatorThermo-Fisher ScientificHeratherm
DimethylformamideMerck Millipore1.10983
Parafilm 'M' laboratory filmBemis #PM992
Tweezers
Needles

Odniesienia

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  2. Muñoz-Espín, D., Serrano, M. Cellular senescence: from physiology to pathology. Nature reviews. Molecular cell biology. 15, 482-496 (2014).
  3. Sharpless, N. E., Sherr, C. J. Forging a signature of in vivo senescence. Nature Reviews Cancer. 15 (7), 397-408 (2015).
  4. Correia-Melo, C., et al. Mitochondria are required for pro-ageing features of the senescent phenotype. The EMBO Journal. 10, e201592862 (2016).
  5. Loaiza, N., Demaria, M. Cellular senescence and tumor promotion: Is aging the key?. Biochimica et Biophysica Acta (BBA) - Reviews on Cancer. , (2016).
  6. Coppe, J. P., et al. Senescence-associated secretory phenotypes reveal cell-nonautonomous functions of oncogenic RAS and the p53 tumor suppressor. PLoS Biology. 6 (12), 2853-2868 (2008).
  7. Baker, D. J., et al. Naturally occurring p16(Ink4a)-positive cells shorten healthy lifespan. Nature. 530 (7589), 184-189 (2016).
  8. Xu, M., et al. Targeting senescent cells enhances adipogenesis and metabolic function in old age. Elife. 4, e12997 (2015).
  9. Baker, D. J., et al. Clearance of p16Ink4a-positive senescent cells delays ageing-associated disorders. Nature. 479 (7372), 232-236 (2011).
  10. Jeon, O. H., et al. Local clearance of senescent cells attenuates the development of post-traumatic osteoarthritis and creates a pro-regenerative environment. Nature Medicine. 23 (6), 775-781 (2017).
  11. Demaria, M., et al. Cellular Senescence Promotes Adverse Effects of Chemotherapy and Cancer Relapse. Cancer Discovery. 7 (2), 165-176 (2017).
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