Low-Cost Automated Flight Intercept Trap for the Temporal Sub-Sampling of Flying Insects Attracted to Artificial Light at Night

Podsumowanie

To study the impacts of artificial light at night (ALAN) on nocturnal flying insects, sampling needs to be confined to nighttime. The protocol describes a low-cost automated flight intercept trap that allows researchers to sample at user-defined periods with increased replication.

Streszczenie

Sampling methods are selected depending on the targeted species or the spatial and temporal requirements of the study. However, most methods for passive sampling of flying insects have a poor temporal resolution because it is time-consuming, costly and/or logistically difficult to perform. Effective sampling of flying insects attracted to artificial light at night (ALAN) requires sampling at user-defined time points (nighttime only) across well-replicated sites resulting in major time and labor-intensive survey effort or expensive automated technologies. Described here is a low-cost automated intercept trap that requires no specialist equipment or skills to construct and operate, making it a viable option for studies that require temporal sub-sampling across multiple sites. The trap can be used to address a wide range of other ecological questions that require a greater temporal and spatial scale than is feasible with previous trap technology.

Wprowadzenie

There are many arthropod sampling techniques^1,2,3, but ecologists often have difficulty applying these methods in ways that are appropriate to their research questions (see⁴). When choosing an appropriate method for sampling insects, ecologists must consider the targeted species, time, effort, and cost involved in different techniques. For example, a common limitation is that it can be logistically challenging to sub-sample during specific time periods over replicated sites to quantify temporal variables which influence species activity, such as changes in weather or circadian activity (but see⁵). Most passive-survey insect traps are set for long periods (e.g., over multiple days, weeks, or even months), lacking fine-scale temporal resolution¹. For surveys targeting specific time periods across multiple replicate sites (such as nocturnal sampling only across distinct sites), a large team may be required to visit sites over multiple days at the same time points (e.g., within 30 min of sunrise and sunset) to collect specimens and reset traps⁶; otherwise, an automated trapping device is required^5,7,8.

There is a growing field of work on the impacts of artificial light at night (ALAN) on insect activity patterns and localized population dynamics^9,10; and on the interactions between ALAN and rates of insect predation^4,11,12,13. However, to study the impacts of ALAN on nocturnal insect taxa, sampling needs to be confined to nighttime. Several different active light traps have been described and used for automated temporal sampling of nocturnal insects¹⁴. Some examples include simple falling disk-type separation devices, where the catch falls into a narrow tube with a disk falling every hour to separate the catch¹⁵, or turn-table separation devices that rotate collection bottles at timed intervals^7,16,17. These previous automated light traps address the sampling challenges involved with temporal survey requirements but are often large and unwieldy and use outdated or unreliable technology. A new automated passive sampling device was recently developed and tested⁸. This device utilized a commercially available flight-interception trap paired with a lightweight custom-designed collection device consisting of a turn-table holding sampling cup that allows for collecting trap contents at user-defined intervals⁸. This new automated trap employs sophisticated programming that can be operated by a smartphone but is prohibitively expensive to build at around EURO 700 (AUD 1,000) per trap⁸.

Flight intercept traps are one of the most efficient ways to survey flying insects^1,18,19 and work on the principle that flying insects fall to the ground when they collide with a vertical surface. Flight intercept traps come in a variety of designs. However, most are typically constructed with a transparent or mesh surface and a collecting container filled with water and/or a preservative. The new trap described here uses a cross vane/baffle type or multidirectional intercept trap²⁰, given that cross baffles have been shown to increase capture rates^14,21 and sample insects from all directions. The purpose of this trap is to survey nocturnal flying insects that are attracted to artificial lights. This phototaxis results in insects circling the light source²²; hence a multidirectional trap is most suitable.

Described here is a low-cost automated intercept trap that requires no specialist equipment or skills to construct and operate. The trap uses a commercially available automated pet food dispenser and common items available from hardware stores. This design costs less than EURO 66 (AUD 105) per trap to construct (Table 1), making them a viable option for studies requiring temporal sub-sampling across multiple sites simultaneously.

Protokół

1. Trap construction

NOTE: All of the components required to build the traps can be found in the Table of Materials. Each trap was constructed as shown in Figure 1 and Figure 2 by one person within 2 h.

1. Use a jigsaw to cut the polycarbonate roofing sheets (8 mm x 610 mm x 2400 mm) into 610 mm x 230 mm sections (Figure 1, item 1 & 2). Then cut a 8 mm center groove halfway up the center of each (610 mm x 230 mm) pane to allow the two panes to slide together to form a cross baffle.
2. Slide the crossed baffles snuggly into the plastic funnel opening (Figure 1, item 4) and secure them to the funnel with 20 mm stainless steel angle brackets (Fig 1, item 5b).
3. With the angle bracket in place, pre-drill holes and then use M4 screws and nuts with washers (Figure 1, item 6b) to secure the cross baffles to the funnel.
4. Again using the jigsaw, cut a piece of the polycarbonate sheeting (230 mm x 305 mm) from remaining sheets and secure with 20 mm stainless steel angle brackets (Figure 1, item 5a) at a 90° angle to the top of the crossed baffles to form a protective roof (Figure 1, item 3).
5. With the angle bracket in place, pre-drill holes and then use M4 screws and nuts with washers (Figure 1, item 6a) to secure the roof to the baffle.
6. Trim the funnel spout to a length of ~30 mm with a hacksaw to ensure the sample trays of the automated pet feeder will rotate unobstructed at the programmed intervals.
7. Place the automated pet dispenser (Figure 1, item 8) within a 9 L (38 mm diameter) plastic basin (Figure 1, item 7) to protect the samples from weather conditions.
8. Drill a 20 mm hole into the top of the 9 L basin and place the funnel spout into the hole to position it directly above the sample tray.
9. Using a drill with a hex-head driver bit, secure the plastic basin covering the pet feeder to a 500 mm piece of treated pine fence paling (Figure 1, item 9) with galvanized hex-head screws (Figure 1, item 14).
10. To stabilize the entire trap for hoisting into the air via ropes, attach a wooden stake (17 x 17 x 1200 mm, Figure 1, item 10) to the piece of treated pine fence paling (Figure 1, item 9) with an angle bracket and tie wire (Figure 1, items 13, 15 and 16).

Figure 1: Schematic diagram of trap construction. (1 and 2) 610 mm x 230 mm x 8 mm polycarbonate sheets; (3) 230 mm x 305 mm x 8 mm polycarbonate sheet; (4) 24 cm diameter plastic funnel; (5a-b) 20 mm angle brackets; (6a-b) M4 x 15 mm screws, washers & nuts; (7) 38 cm diameter 9 L plastic basin; (8) automated pet food dispenser; (9) 150 mm x 12 mm treated pine paling; (10) 17 mm x 17 mm x 1200 mm wooden stake; (11) 125 mm x 150 mm angle bracket; (12) 16 mm x 16 mm hex-head screws; (13) angle bracket; (14) 16 mm x 16 mm hex-head screws; (15 and 16) wire stabilizer; (17) karabiner, used for lowering and raising into position. Please click here to view a larger version of this figure.

2. Trap deployment

NOTE: The traps were attached to trees 6 m above the ground (directly below the experimental or control lights) to capture flying insects (Figure 2). Emptying and collection of traps were done by three people on a single day. Further days can be sampled if required by lowering the trap to remove the collected samples, resetting the pet food dispensers, and placing the trap back in position every three days based on the sampling regime.

Figure 2: Low-cost automated flight intercept trap for sampling insects at user-defined time points. (A) Polycarbonate crossed baffles serve as the flight intercept area that allows for collecting insects from all four sides. The polycarbonate roof serves to direct insects downwards and protect the collected samples from the weather. The funnel beneath the flight intercept barrier servers to funnel insects that have collided with the polycarbonate barriers into the collecting trays housed within the circular basin. (B) Trap suspended below experimental light and secured to the tree by a wooden stake and angle bracket. The plywood box below the intercept trap contains a bat detector used to passively record echolocation calls produced by free-ranging insectivorous bats. Please click here to view a larger version of this figure.

1. Once at the sampling location, remove the automated pet food dispenser (Figure 1, item 8) from beneath the plastic basin.
2. Open up the automated pet food dispenser (Figure 3A) and place foil dishes containing soapy water or a preservative of choice in each food tray (Figure 3B, here 20 mL of propylene glycol was used as a preservative).
3. Follow the directions provided with the automated pet food dispenser to set the food tray rotation times. First, set the clock time, then program each pet food dispenser tray.
   ​NOTE: The automated pet food dispenser rotates food trays (1-6) at pre-programmed times. The 6-meal bowls can be set to open at any time of the day or night, with the trays rotating in sequential order. For this study, the aim was to sample nocturnal and diurnal insects separately. Tray 1 sampled from 8 PM on the first night and then moved to tray 2 at 7 AM the following morning, followed by tray 3 at 8 PM, tray 4 at 7 AM, tray 5 at 8 PM and tray 6 at 7 AM. A delay function allowed for a one-day delay in sampling because it took 2 days to set up all sites, thereby ensuring sampling commenced on the same day/time at all sites.

Figure 3: Automated pet food bowl. (A) Battery-operated 6-meal pet food bowl used to sample insects at user-defined intervals. The food bowls were programmed on alternating schedules to sample nocturnal and diurnal insects. For example, tray 1 opened at 8 PM (nocturnal day 1), tray 2 opened at 7 AM (diurnal day 1), tray 3 opened at 8 PM (nocturnal day 2), tray 4 opened at 7 AM (diurnal day 2), tray 5 opened at 8 PM (nocturnal day 3), and tray 6 opened at 7 AM (diurnal day 3). (B) Lid removed from automated pet food bowl to show the six collection trays. Foil dishes containing propylene glycol as a preservative allowed for the easy removal of the collected insects. Please click here to view a larger version of this figure.

4. Place the automated pet food dispenser back underneath the plastic basin and secure the basin to the timber fence piece with the galvanized hex-head screws (Figure 1, item 14).
5. Attach a rope to the top of the trap with a karabiner (Figure 1, item 17). With the use of a ladder, hoist the trap into position and secure it beneath experimental lights by the karabiner.
6. Attach a second wooden stake (17 mm x 17 mm x 1200 mm, Figure 2B) to the tree (or lamp post) with an angle bracket to stabilize the trap in high winds.
7. The trap sits on top of the stake; secure it with two large cable ties (Figure 2B).
8. To collect insect samples, lower the traps with a rope. Remove the automated pet food dispenser from beneath the plastic basin.
9. Remove the lid of the pet food dispenser (Figure 3A) and lift the aluminum trays out to pour the contents into pre-labeled sample vials (Figure 3B).

Wyniki

The traps were trialed in a survey of flying insects attracted to experimental lighting at four bushland reserves across Melbourne, Australia. Sites consisted of either remnant or revegetated bushland surrounded by residential housing and averaged 15 km apart (range 3-24 km) and 45 ha in size (range 30-59 ha). A total of sixteen traps were installed, four at each site, with and without experimental lights (3 lights and 1 control per site), and surveyed for 3 days and 3 nights from 30 March to 2 April 2021. Installing the...

Dyskusje

Despite the automated flight-intercept trap described by Bolliger et al. (2020)⁸ being well designed and very effective at sampling at user-defined time periods, they are likely to be cost-prohibitive for many researchers. This study shows that passive trapping surveys using automated traps for sub-sampling flying insects at user-defined periods can be carried out on a modest budget. Traps were built to sample at six pre-defined time points by utilizing a commercial pet food dispenser and material...

Materiały

Name	Company	Catalog Number	Comments
Batteries (C cell) – 10 pack	Duracell	MN1400B10	https://www.duracell.com.au/product/alkaline-c-batteries/
Battery operated automated 6 meal pet food bowl – each	OEM China	XR-P006-002	Automated 6-meal pet food bowls range in price dependent on supplier, for example in the UK they can be purchased for £19 GBP ($36 AUD).
Galvanised hex-head screws (10-16 x 16 mm) – 100 pack	Bunnings Warehouse	1-311-9151-CTPME	Bunnings Warehouse is an Australian hardware chain with stores in Australia and New Zealand. Items purchased from Bunnings Warehouse can be found at most hardware stores. https://www.bunnings.com.au/
Galvanised steel angle bracket (125 x 150 mm) – each	Bunnings Warehouse	AZ11	https://www.bunnings.com.au/
Galvanised tie wire (0.70 mm x 75 m) – per roll	Bunnings Warehouse	50218	https://www.bunnings.com.au/
Plastic basin (38 cm, 9 L round) – each	Ezy Storage	FBA31541	https://www.ezystorage.com/product/laundry/basic-accessories/9l-round-basin/
Plastic funnel (24 cm) – each	Sandleford	Pf24	https://www.sandleford.com.au/plastic-funnel-24cm
Stainless steel angle bracket (20 mm) – 16 pack	Bunnings Warehouse	WEB2020	https://www.bunnings.com.au/
Stainless steel screws & nuts (M4 x 15 mm) – 18 pack	Bunnings Warehouse	SFA394	https://www.bunnings.com.au/
Stainless steel washers (3/16” & M5) – 50 pack	Bunnings Warehouse	EBM5005	https://www.bunnings.com.au/
Sunlite Polycarbonate roofing sheet (8mm x 610 mm x 2.4 m) – each	Suntuf (Palram Industries Ltd)	SL8CL2.4	https://www.palram.com/au/product/sunlite-polycarbonate-multi-wall/
Treated pine paling (150 x 12 mm) – each	STS Timber Wholesale P/L	n/a	https://www.ststimber.com.au/sts-timber-wholesale-products/fencing
Wooden stakes (1200 x 17 x 17 mm) – 10 pack	Lattice Makers	n/a	https://latticemakers.com/product/tomato-stakes-17x17mm-pack-of-10/