To begin, incubate two milliliters of the cultured MCF-7 cells in a six-well plate until 90%confluency. In the grown cell monolayer, create a linear scratch wound down the center using a sterile pipette tip. Then capture the images with an optical microscope at different time intervals.
For the transwell assay, suspend MCF-7 cells in a serum-free medium. Then see the cells in the upper transwell chamber, either pre-coded or non-coded with Matrigel. Add DMEM complete medium at the bottom of the transwell chamber to act as a chemical inducer.
After 24 hours, remove the cells in the upper chamber and fix the remaining invasive and migrant cells using methanol. Stain the MCF-7 cells using crystal violet solution. Next, image the stained cells with an optical microscope.
A dose dependent inhibitory effect of salidroside on cell proliferation was observed, with a 50%decline in cell vitality at 40 micromolar. Further, salidroside could inhibit the vitality of MCF-7 cells over time, with a 50%decrease in MCF-7 cell vitality after 24 hours of co-incubation. The wound-scratch assay showed an inhibitory effect for salidroside treatment on MCF-7 cells.
Further, salidroside treatment significantly reduced the undesirable migration and invasion of MCF-7 cells.
