探究红景天苷抑制MCF-7细胞增殖和迁移的药理作用及分子机制

1.9K Views

11:13 min

June 9th, 2023

DOI :10.3791/65634-v

June 9th, 2023

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MCF 7 MCF 7 PI3K AKT HIF 1 FoxO1 CCK 8 ROS Ca2 Na K ATP Ca2 ATP QRT PCR

Chapters in this video

0:00

Introduction

1:47

Evaluating the Migration and Invasion of Salidroside Treated MCF‐7 Cells in a Wound Scratch Assay

3:24

Determining Na+-K+-ATPase and Ca2+-Mg2+-ATPase Activity in Salidroside Treated MCF‐7 Cells

4:12

Flow Cytometry Analysis of Apoptosis and the Cell Cycle in Salidroside Treated MCF‐7 Cells

5:07

DCFH‐DA and Fluo‐4 AM Fluorescence Staining of Salidroside Treated MCF‐7 Cells

6:23

Analyzing Protein Expression in Salidroside Treated MCF‐7 Cells by Western Blotting

8:45

Determining Gene Expression in Salidroside Treated MCF‐7 Cells Using qRT‐PCR

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