Isolation, Expansion, and Nucleofection of Neural Stem Cells from Adult Murine Subventricular Zone

Please note that all translations are automatically generated. Click here for the English version.

2.0K Views

•

09:19 min

•

June 14th, 2024

DOI :

10.3791/66651-v

June 14th, 2024

•

Esteban Jiménez-Villalba*¹, Laura Lázaro-Carot*¹, Carmen M. Mateos-Martínez¹, Jennifer Díaz-Moncho¹, Daniel Samper-Llavador¹, Marta Igual-López¹, Jordi Planells¹, Sacri R. Ferrón¹

¹Instituto de Biotecnología y Biomedicina (BIOTECMED), Departamento de Biología Celular, Biología Funcional y Antropología Física, Universitat de València

Podsumowanie

Przeglądaj więcej filmów

Central Nervous System

Basic Fibroblast Growth Factor bFGF

Epidermal Growth Factor EGF

Neurospheres

SiRNA Delivery

Gene Delivery Efficiency

Cell Viability

Rozdziały w tym wideo

0:00

Introduction

1:35

Murine Brain Extraction and Subventricular Zone (SVZ) Dissection for Isolation of Neural Stem Cells

3:42

Isolation and Seeding of Murine Subventricular Zone‐Derived Neural Stem Cells (NSCs) for Neurosphere Formation

5:54

Expansion of Neurospheres Obtained from Murine Subventricular Zone‐Derived Neural Stem Cells

7:33

Nucleofection of Neurosphere Cultures Obtained from Murine Subventricular Zone‐Derived Neural Stem Cells

Powiązane Filmy

14:33

The Subventricular Zone En-face: Wholemount Staining and Ependymal Flow

41.7K Views

07:00

Isolation and Culture of Neural Crest Stem Cells from Human Hair Follicles

16.8K Views

09:10

Preparation of Acute Subventricular Zone Slices for Calcium Imaging

13.1K Views

19:45

Expansion of Embryonic and Adult Neural Stem Cells by In Utero Electroporation or Viral Stereotaxic Injection

16.7K Views

12:48

Isolation and Culture of Neural Crest Cells from Embryonic Murine Neural Tube

17.1K Views

08:06

Neonatal Subventricular Zone Electroporation

12.7K Views

11:27

Growing Neural Stem Cells from Conventional and Nonconventional Regions of the Adult Rodent Brain

12.1K Views

09:37

A cGMP-applicable Expansion Method for Aggregates of Human Neural Stem and Progenitor Cells Derived From Pluripotent Stem Cells or Fetal Brain Tissue

13.4K Views

09:52

One Mouse, Two Cultures: Isolation and Culture of Adult Neural Stem Cells from the Two Neurogenic Zones of Individual Mice

37.5K Views

09:27

Cell Sorting of Neural Stem and Progenitor Cells from the Adult Mouse Subventricular Zone and Live-imaging of their Cell Cycle Dynamics

14.1K Views