Calculating the O₂ Concentration at the RPE Monolayer to Determine if Cells are in Hypoxic, Normoxic, or Hyperoxic Conditions

To begin, monitor oxygen consumption rates, or OCR, in well-differentiated RPE cultures in a standard cell culture incubator using Resipher system. After the experiment, open the calculator to estimate oxygen levels at the cell monolayer and enter the media volume in microliters. Enter the saturated oxygen concentration at the air liquid interface.

In the flux input, enter the flux or OCR reported by the system. Based on these values, the steady state oxygen concentration at each height in the media column is calculated and shown in the plot. The X axis of the plot represents the height in millimeters above the bottom of the well.

The Y axis represents the calculated oxygen concentration at that height. The oxygen concentration at the bottom of the well is reported in the line below the plot.