Long-term Monitoring of Oxygen Consumption Rates in Highly Differentiated and Polarized Retinal Pigment Epithelial Cultures
4.9K Views
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08:09 min
•
August 16th, 2024
DOI :
August 16th, 2024
•Qitao Zhang*1, Daisy Y. Shu*2, Richard A. Bryan3, John Y. S. Han1, Gillian A. Gulette1, Kin Lo3, Leo A. Kim4, Jason M. L. Miller1,5
1Kellogg Eye Center, University of Michigan, Ann Arbor, 2School of Optometry and Vision Science, University of New South Wales, 3Lucid Scientific, 4Schepens Eye Research Institute of Mass. Eye and Ear, Department of Ophthalmology, Harvard Medical School, 5Cellular and Molecular Biology Program, University of Michigan, Ann Arbor
Chapters in this video
0:00
Introduction
1:49
Setup of Resipher System "Sandwich" to Monitor Oxygen Consumption Rate in Well‐Differentiated RPE Cultures
5:11
Measuring Changes in Mitochondrial Metabolism in Hypoxic RPE Using Resipher System
7:03
Calculating the O2 Concentration at the RPE Monolayer to Determine if Cells are in Hypoxic, Normoxic, or Hyperoxic Conditions
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