Detection of Bacteria Using Fluorogenic DNAzymes

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18.9K Views

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13:20 min

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May 28th, 2012

DOI :

10.3791/3961-v

May 28th, 2012

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Sergio D. Aguirre¹, M. Monsur Ali¹, Pushpinder Kanda¹, Yingfu Li¹^,²

¹Department of Biochemistry and Biomedical Sciences, McMaster University , ²Department of Chemistry and Chemical Biology, McMaster University

Podsumowanie

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Hospital acquired Pathogens

Short Time to results

Operational Simplicity

Cost Effectiveness

Classical Microbiological Methods

PCR based Techniques

Antibody based Techniques

Scientific Research

Rozdziały w tym wideo

0:05

Title

2:02

Construction of RFD-EC1 and RFSS1 by Template Mediated Enzymatic Ligation

4:38

Detection using Fluorescence Spectrophotometer

6:28

Detection by Gel Electrophoresis

8:04

Single Cell Detection

9:55

Experimental Validation using E. coli Crude Extracellular Mixtures

12:42

Conclusion

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