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Detection of Bacteria Using Fluorogenic DNAzymes Video (Video) | JoVE

DOI :

10.3791/3961-v

13:20 min

May 28th, 2012

May 28th, 2012

18,254 Views

1Department of Biochemistry and Biomedical Sciences, McMaster University , 2Department of Chemistry and Chemical Biology, McMaster University

We have recently reported a novel approach for generating fluorogenic DNAzyme probes that can be applied to set up a simple, "mix-and-read" fluorescent assay for bacterial detection. These special DNA probes catalyze the cleavage of a chromophore-modified DNA-RNA chimeric substrate in the presence of crude extracellular mixture (CEM) produced by a specific bacterium, thereby translating bacterial detection into fluorescence signal generation. In this report we will describe key experimental procedures where a specific DNAzyme probe denoted "RFD-EC1" is employed for the detection of the model bacterium, Escherichia coli (E. coli).

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Bacteria Detection

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