Ex Vivo Red Blood Cell Hemolysis Assay for the Evaluation of pH-responsive Endosomolytic Agents for Cytosolic Delivery of Biomacromolecular Drugs
Please note that all translations are automatically generated. Click here for the English version.
34.3K Views
•
11:12 min
•
March 9th, 2013
DOI :
March 9th, 2013
•Brian C. Evans*1,2, Christopher E. Nelson*1,2, Shann S. Yu*1,2, Kelsey R. Beavers2,3, Arnold J. Kim1, Hongmei Li1,2, Heather M. Nelson4, Todd D. Giorgio1,2,5,6, Craig L. Duvall1,2
1Department of Biomedical Engineering, Vanderbilt University, 2Vanderbilt Institute for Nanoscale Science & Engineering, Vanderbilt University, 3Interdisciplinary Materials Science Program, Vanderbilt University, 4Monroe Carell Jr. Children's Hospital, Vanderbilt University Medical Center, 5Department of Chemical & Biomolecular Engineering, Vanderbilt University, 6Department of Cancer Biology, Vanderbilt University
Rozdziały w tym wideo
0:26
Introduction fo Endosomolysis and Hemolysis
2:16
Preparation of Buffers and Test Reagents
3:14
Blood Sample Preparation
6:05
Assay Setup and Quantification
8:39
Data Analysis
9:00
Representative Results
9:25
Discussion
0:01
Title
Powiązane Filmy
09:57
A Facile and Efficient Approach for the Production of Reversible Disulfide Cross-linked Micelles
8.8K Views
12:15
Single Liposome Measurements for the Study of Proton-Pumping Membrane Enzymes Using Electrochemistry and Fluorescent Microscopy
7.4K Views
09:15
Induction of Eryptosis in Red Blood Cells Using a Calcium Ionophore
8.7K Views
13:55
Measurement of Vacuolar and Cytosolic pH In Vivo in Yeast Cell Suspensions
19.8K Views
07:31
Measuring the pH, Redox Chemistries, and Degradative Capacity of Macropinosomes using Dual-Fluorophore Ratiometric Microscopy
2.3K Views
09:46
Simultaneous pH Measurement in Endocytic and Cytosolic Compartments in Living Cells using Confocal Microscopy
14.9K Views
05:16
A Photodynamic Approach to Study Function of Intracellular Vesicle Rupture
1.1K Views
09:48
In vitro Quantitative Imaging Assay for Phagocytosis of Dead Neuroblastoma Cells by iPSC-Macrophages
5.4K Views
11:37
Cellular Redox Profiling Using High-content Microscopy
10.9K Views
01:46
Test hemolizy: metoda in vitro do pomiaru lizy indukowanej jonoforem wapnia w ludzkich erytrocytach
736 Views
Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone