In Vivo Modeling of the Morbid Human Genome using Danio rerio

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20.5K Views

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12:31 min

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August 24th, 2013

DOI :

10.3791/50338-v

August 24th, 2013

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Adrienne R. Niederriter¹^,², Erica E. Davis¹^,³, Christelle Golzio¹, Edwin C. Oh¹, I-Chun Tsai¹, Nicholas Katsanis¹

¹Center for Human Disease Modeling, Department of Cell Biology, Duke University Medical Center, ²Department of Evolutionary Anthropology, Duke University, ³Department of Pediatrics, Duke University Medical Center

Podsumowanie

Rozdziały w tym wideo

0:05

Title

1:20

Bioinformatics and Expressions Analysis in the Developing Zebrafish Embryo

2:27

Site-directed Mutagenesis and in vitro mRNA Transcription

3:54

In vivo Assay of Variant Loss of Function

6:40

In vivo Assay of Variant Dominant Negative or Gain of Function Effects

8:11

Representative MO Knockdown and Rescue Results

12:06

Conclusion

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