August 24th, 2013
•Here, we present a systematic approach for developing physiologically relevant, sensitive and specific in vivo assays for interpreting variation in human pathology. Transient genetic manipulation via microinjection of WT and mutant human mRNA and morpholino (MO) antisense oligonucleotides harness the tractability of the developing zebrafish embryo to rapidly assay pathogenic mutations, especially, but not exclusively, in the context of human developmental disorders.
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Gross and Fine Dissection of Inner Ear Sensory Epithelia in Adult Zebrafish Danio rerio Video (Video) | JoVE
Flow Cytometry Purification of Mouse Meiotic Cells Video (Video) | JoVE
Heart Dissection in Larval, Juvenile and Adult Zebrafish, Danio rerio Video (Video) | JoVE
Regular Care and Maintenance of a Zebrafish Danio rerio Laboratory: An Introduction Video (Video) | JoVE
High Throughput Danio Rerio Energy Expenditure Assay Video (Video) | JoVE
Genome-wide Mapping of Drug-DNA Interactions in Cells with COSMIC Crosslinking of Small Molecules to Isolate Chromatin Video (Video) | JoVE
Gap Junctional Intercellular Communication: A Functional Biomarker to Assess Adverse Effects of Toxicants and Toxins, and Health Benefits of Natural Products Video (Video) | JoVE
In Vivo Study of Human Endothelial-Pericyte Interaction Using the Matrix Gel Plug Assay in Mouse Video (Video) | JoVE
Measuring the Stiffness of Ex Vivo Mouse Aortas Using Atomic Force Microscopy Video (Video) | JoVE
In Vitro and In Vivo Approaches to Determine Intestinal Epithelial Cell Permeability Video (Video) | JoVE