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Detecting, Visualizing and Quantitating the Generation of Reactive Oxygen Species in an Amoeba Model System

16.1K Views

16:41 min

November 5th, 2013

DOI :

10.3791/50717-v

November 5th, 2013


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Keywords Reactive Oxygen Species

Rozdziały w tym wideo

0:05

Title

2:42

OxyBurst Green (OBG)-Coating of Beads

5:43

Visualization and Qualitative Measurement of ROS Production in Phagosomes

8:48

Quantitative and Medium-throughput Measurement of Intracellular Superoxide Production

10:30

Quantitative and Medium-throughput Measurement of Extracellular H2O2 Production

12:04

Results: OxyBurst Green (OBG), Dihydroethidium (DHE) and Amplex UltraRed (AUR) Assays can Detect and Measure ROS in Dictyostelium

15:54

Conclusion

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