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Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses

21.3K Views

12:20 min

December 29th, 2015

DOI :

10.3791/53164-v

December 29th, 2015


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Bacterial Artificial Chromosomes

Rozdziały w tym wideo

0:05

Title

1:18

Summary of Procedures in Video Format

1:51

High-purity Maxi-prep of Full-length SA14-14-2 BAC: Part 1

4:54

High-purity Maxi-prep of Full-length SA14-14-2 BAC: Part 2

6:48

Transcribe Synthetic RNAs In Vitro from a Linearized Full-length JEV BAC DNA

8:55

Determine RNA Infectivity and Virus Yield

10:36

Results: Specific Infectivity of the Synthetic RNAs Transcribed from a Full-length JEV BAC and the Recovery of Synthetic Virus

11:40

Conclusion

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