Bacterial Artificial Chromosomes: uno strumento Genomica Funzionale per lo studio di positivo-strand virus RNA

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Summary of Procedures in Video Format

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High-purity Maxi-prep of Full-length SA₁₄-14-2 BAC: Part 1

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High-purity Maxi-prep of Full-length SA₁₄-14-2 BAC: Part 2

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Transcribe Synthetic RNAs In Vitro from a Linearized Full-length JEV BAC DNA

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Determine RNA Infectivity and Virus Yield

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Results: Specific Infectivity of the Synthetic RNAs Transcribed from a Full-length JEV BAC and the Recovery of Synthetic Virus

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Conclusion