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Examination of Proteins Bound to Nascent DNA in Mammalian Cells Using BrdU-ChIP-Slot-Western Technique

9.1K Views

09:14 min

January 14th, 2016

DOI :

10.3791/53647-v

January 14th, 2016


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BrdU ChIP Slot Western

Rozdziały w tym wideo

0:05

Title

0:44

Chromatin Preparation with BrdU Labeling

3:06

Chromatin Immunoprecipitation (ChIP)

5:28

Slot Blot Analysis of Chip DNA

7:22

Results: Loss of Histone Deacetylase 1 and 2 Increases H4K16ac on Nascent DNA and SMARCA5 Associates with Nascent DNA

8:21

Conclusion

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