Results: Loss of Histone Deacetylase 1 and 2 Increases H4K16ac on Nascent DNA and SMARCA5 Associates with Nascent DNA
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Conclusion
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The overall goal of this procedure is to rapidly examine proteins bound to nascent DNA with accuracy using BrdU labeling and chromatin precipitation. This method can answer key questions in the replication, repair in chromatin field, such as, what
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In this protocol, we describe a novel BrdU-ChIP-Slot-Western technique to examine proteins and histone modifications associated with newly synthesized or nascent DNA.