JoVE Logo

Zaloguj się

Protein Complex Affinity Capture from Cryomilled Mammalian Cells

14.9K Views

10:37 min

December 9th, 2016

DOI :

10.3791/54518-v

December 9th, 2016


Przeglądaj więcej filmów

Protein Complex

Rozdziały w tym wideo

0:05

Title

0:30

Cell Harvesting and Freezing

3:09

Cryogenic Disruption of Frozen Cells

4:50

Affinity Capture of Protein Complexes from Cell Extracts

6:02

Affinity Capture

7:41

Results: Comparing Some Different Aspects of Affinity Capture Optimization

9:38

Conclusion

Powiązane Filmy

article

10:53

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution

9.0K Views

article

09:35

Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling

13.8K Views

article

09:27

Adaptation of Hybridization Capture of Chromatin-associated Proteins for Proteomics to Mammalian Cells

6.2K Views

article

09:33

High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis

7.1K Views

article

10:50

Isolation of Labile Multi-protein Complexes by in vivo Controlled Cellular Cross-Linking and Immuno-magnetic Affinity Chromatography

17.3K Views

article

08:02

Extracting Modified Microtubules from Mammalian Cells to Study Microtubule-Protein Complexes by Cryo-Electron Microscopy

1.4K Views

article

06:45

Dissecting Multi-protein Signaling Complexes by Bimolecular Complementation Affinity Purification (BiCAP)

7.4K Views

article

10:02

Identification of Protein Interacting Partners Using Tandem Affinity Purification

37.4K Views

article

14:58

Identification of Protein Complexes in Escherichia coli using Sequential Peptide Affinity Purification in Combination with Tandem Mass Spectrometry

48.2K Views

article

13:51

The MultiBac Protein Complex Production Platform at the EMBL

15.9K Views

JoVE Logo

Prywatność

Warunki Korzystania

Zasady

Badania

Edukacja

O JoVE

Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone