Crystal Structure of the N-terminal Domain of Ryanodine Receptor from Plutella xylostella

Please note that all translations are automatically generated. Click here for the English version.

7.4K Views

•

11:31 min

•

November 30th, 2018

DOI :

10.3791/58568-v

November 30th, 2018

•

Bidhan Chandra Nayak*¹, Jie Wang*¹, Lianyun Lin*¹^,²^,³^,⁴, Weiyi He²^,³^,⁴, Minsheng You²^,³^,⁴, Zhiguang Yuchi¹^,²

¹Tianjin Key Laboratory for Modern Drug Delivery & High-Efficiency, Collaborative Innovation Center of Chemical Science and Engineering, School of Pharmaceutical Science and Technology, Tianjin University, ²State Key Laboratory of Ecological Pest Control for Fujian/Taiwan Crops and Institute of Applied Ecology, Fujian Agriculture and Forestry University, ³Joint International Research Laboratory of Ecological Pest Control, Ministry of Education, Fuzhou, ⁴Fujian-Taiwan Joint Centre for Ecological Control of Crop Pests, Fujian Agriculture and Forestry University

Podsumowanie

Przeglądaj więcej filmów

Keywords Ryanodine Receptor

X ray Crystallography

Protein Crystallography

Polymerase Chain Reaction

DNA Amplification

LIC Vector

T4 DNA Polymerase

Ligation independent Cloning

BL21 DE3 E Coli

Protein Expression

Rozdziały w tym wideo

0:04

Title

1:01

Ligation-independent Cloning (LIC)

2:38

E. coli Transformation and Protein Expression

4:58

Fusion Protein Purification, Tag-cleaving, and Target Protein Re-purification

6:12

Protein Crystallization, Crystal Mounting, and Structure Determination

9:56

Results: Representative N-terminal Domain (NTD) Crystal Structure Determination

10:53

Conclusion

Powiązane Filmy

11:30

Tandem Affinity Purification of Protein Complexes from Eukaryotic Cells

14.8K Views

09:18

Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics

9.8K Views

14:25

Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain

17.7K Views

12:26

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

18.4K Views

06:01

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions

7.1K Views

05:27

Immunization of Alpacas (Lama pacos) with Protein Antigens and Production of Antigen-specific Single Domain Antibodies

23.3K Views

06:42

Structure Solution of the Fluorescent Protein Cerulean Using MeshAndCollect

5.6K Views

09:52

Probing The Structure And Dynamics Of Nucleosomes Using Atomic Force Microscopy Imaging

11.5K Views

09:24

Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy

7.9K Views

13:02

Production, Crystallization, and Structure Determination of the IKK-binding Domain of NEMO

7.4K Views