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Sample Preparation for Mass-spectrometry-based Proteomics Analysis of Ocular Microvessels
11.2K Views
•
11:32 min
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February 22nd, 2019
DOI :
February 22nd, 2019
•Rozdziały w tym wideo
0:04
Title
1:02
Isolation of Short Posterior Ciliary Arteries
2:17
Sample Preparation
3:43
Pellet Digestion
4:40
Sample Cleaning and Buffer Exchange
5:40
One-dimensional Gel Electrophoresis (1DE)
6:50
In-gel Tryptic Digestion
9:23
Results: Comparison of Protein Extraction Efficacy and Robustness and Representative 1DE Gel Analysis
10:51
Conclusion
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