Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models
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9.4K Views
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13:47 min
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March 29th, 2019
DOI :
March 29th, 2019
•Rozdziały w tym wideo
0:04
Title
2:01
Plasmid Design and Construction
3:20
HEK-293T Cells Culturing, Plating, and Transfection
5:15
Virus Harvesting and Viral Particles Concentrating
8:16
Transduction of MD NPCs
9:48
Analysis of Methylation Changes
11:04
Results: Assessment of the LV-dCas9-DNMT3A-GFP/Puro Vectors Compared to the Naive GFP Counterpart
12:45
Conclusion
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