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10:21 min
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June 20th, 2019
DOI :
June 20th, 2019
•0:04
Title
1:12
Expression of FAHD Proteins in Competent E. coli
3:44
Lysis of Bacterial Pellets and Filtration of Debris
4:33
Purification of His-tagged FAHD Proteins using Ni-NTA Affinity Chromatography
5:59
Purification of FAHD Proteins via Ion Exchange Chromatography
6:35
Basic FAHD Activity Assays with Substrates Oxaloacetate and Acetylpyruvate
7:53
Results: Expression and Purification of Fumarylacetoacetate Hydrolase Domain-containing Proteins
9:19
Conclusion
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