Single-Step Enrichment of a TAP-Tagged Histone Deacetylase of the Filamentous Fungus Aspergillus nidulans for Enzymatic Activity Assay

Please note that all translations are automatically generated. Click here for the English version.

6.7K Views

•

09:07 min

•

May 1st, 2019

DOI :

10.3791/59527-v

May 1st, 2019

•

Ingo Bauer¹, Angelo Pidroni¹, Özgür Bayram²^,³, Gerald Brosch¹, Stefan Graessle¹

¹Division of Molecular Biology, Biocenter, Medical University of Innsbruck, ²Biology Department, Maynooth University, ³Maynooth University Human Health Research Institute

Podsumowanie

Przeglądaj więcej filmów

Aspergillus Nidulans

TAP tagged Histone Deacetylase

Enzymatic Activity Assay

Class 1 Histone Deacetylases

Deactylase Inhibitors

Protease Inhibitor Cocktail

Rozdziały w tym wideo

0:04

Title

0:53

Inoculum Preparation

1:34

Growth and Harvesting of Mycelia

2:36

Preparation of the Protein Extract and Equilibration of the IgG Resin

4:40

Batch Purification of TAP-tagged HDAC

6:50

Results: Analysis of the Purification and Enzymatic Activity Assay

8:29

Conclusion

Powiązane Filmy

09:52

A Fluorescence-based Assay of Phospholipid Scramblase Activity

13.8K Views

09:47

Analysis of Histone Antibody Specificity with Peptide Microarrays

39.8K Views

09:11

Caffeine Extraction, Enzymatic Activity and Gene Expression of Caffeine Synthase from Plant Cell Suspensions

12.6K Views

08:12

Single-step Purification of Macromolecular Complexes Using RNA Attached to Biotin and a Photo-cleavable Linker

7.2K Views

07:53

A Fluorogenic Peptide Cleavage Assay to Screen for Proteolytic Activity: Applications for coronavirus spike protein activation

33.0K Views

11:54

Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry

9.3K Views

04:30

A Colorimetric Assay of Citrate Synthase Activity in Drosophila Melanogaster

9.9K Views

07:59

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products

9.6K Views

10:28

A Semi-High-Throughput Adaptation of the NADH-Coupled ATPase Assay for Screening Small Molecule Inhibitors

9.4K Views

07:10

Isolation of Histone from Sorghum Leaf Tissue for Top Down Mass Spectrometry Profiling of Potential Epigenetic Markers

4.5K Views