Methods Article

Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter

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8.5K Views

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12:15 min

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May 29th, 2019

DOI :

10.3791/59734-v

May 29th, 2019

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Tiago Franco de Oliveira*¹^,², Antonio Anax Falcão de Oliveira*¹, Miriam Lemos³, Mariana Veras³, Paulo Hilário Nascimento Saldiva³^,⁴, Marisa Helena Gennari de Medeiros⁵, Paolo Di Mascio⁵, Ana Paula de Melo Loureiro¹

¹Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, ²Departamento de Farmacociências, Universidade Federal de Ciências da Saúde de Porto Alegre, ³Laboratório de Poluição Atmosfêrica Experimental - LIM05, Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo, ⁴Instituto de Estudos Avançados, Universidade de São Paulo, ⁵Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo

Podsumowanie

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Tissue Homogenization

Proteinase K

Ribonuclease A

Protein Precipitation

DNA Extraction

Rozdziały w tym wideo

0:04

Title

1:20

DNA Extraction

4:18

Quantification by HPLC-UV and Solid Phase Extraction for Analyses

6:20

Quantification by HPLC-ESI-MS/MS

9:19

Quantification of the DNA Lesions

10:36

Results: HPLC-UV and HPLC-ESI-MS/MS Analyses

11:25

Conclusion

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