Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter
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8.5K Views
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12:15 min
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May 29th, 2019
DOI :
May 29th, 2019
•Tiago Franco de Oliveira*1,2, Antonio Anax Falcão de Oliveira*1, Miriam Lemos3, Mariana Veras3, Paulo Hilário Nascimento Saldiva3,4, Marisa Helena Gennari de Medeiros5, Paolo Di Mascio5, Ana Paula de Melo Loureiro1
1Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, 2Departamento de Farmacociências, Universidade Federal de Ciências da Saúde de Porto Alegre, 3Laboratório de Poluição Atmosfêrica Experimental - LIM05, Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo, 4Instituto de Estudos Avançados, Universidade de São Paulo, 5Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo
Rozdziały w tym wideo
0:04
Title
1:20
DNA Extraction
4:18
Quantification by HPLC-UV and Solid Phase Extraction for Analyses
6:20
Quantification by HPLC-ESI-MS/MS
9:19
Quantification of the DNA Lesions
10:36
Results: HPLC-UV and HPLC-ESI-MS/MS Analyses
11:25
Conclusion
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