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Methods Article

Strategic Screening and Characterization of the Visual GPCR-mini-G Protein Signaling Complex for Successful Crystallization

6.9K Views

09:19 min

March 16th, 2020

DOI :

10.3791/60747-v

March 16th, 2020


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GPCR

Rozdziały w tym wideo

0:04

Introduction

0:45

Cell Membrane Solubilization and Protein Extraction

3:53

UV-VIS Spectroscopy

4:33

Automated Size-Exclusion Chromatography of Rhodopsin and Rhodopsin Mini-Go Complex

6:44

Results: UV-VIS Spectroscopy, SEC, and SDS-PAGE

8:34

Conclusion

6:04

Deglycosylation

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