Measuring Naturally Acquired Phagocytosis-Inducing Antibodies to Plasmodium falciparum Parasites by a Flow Cytometry-Based Assay

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6.3K Views

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09:57 min

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August 6th, 2020

DOI :

10.3791/61538-v

August 6th, 2020

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Maria del Pilar Quintana¹, Nsoh Godwin Anabire¹^,²^,³, Lars Hviid¹^,⁴

¹Centre for Medical Parasitology, Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen, ²West African Centre for Cell Biology of Infectious Pathogens, Department of Biochemistry, Cell and Molecular Biology, University of Ghana, ³Department of Immunology, Noguchi Memorial Institute for Medical Research, ⁴Centre for Medical Parasitology, Department of Infectious Diseases, Rigshospitalet

Podsumowanie

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Phagocytosis

Plasmodium Falciparum

Parasite Culture Medium

Immunization

Natural Exposure

Rozdziały w tym wideo

0:04

Introduction

0:33

THP-1 and Infected Erythrocyte (IE) Culture Setup

1:58

Parasite Staining and Opsonization Plate Preparation

3:47

THP-1 Cell and Phagocytosis Plate Preparation

4:35

Phagocytosis Assay

6:21

Flow Cytometry Acquisition and Analysis

7:34

Results: Representative Evaluation of Parasite-IE Opsonization and Phagocytosis

9:21

Conclusion

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