Isolation of High Quality Murine Atrial and Ventricular Myocytes for Simultaneous Measurements of Ca2+ Transients and L-Type Calcium Current
Please note that all translations are automatically generated. Click here for the English version.
3.0K Views
•
06:22 min
•
November 3rd, 2020
DOI :
November 3rd, 2020
•Philipp Tomsits*1,2,3, Dominik Schüttler*1,2,3, Stefan Kääb1,2, Sebastian Clauss*1,2,3, Niels Voigt*4,5,6
1Department of Medicine I, University Hospital Munich, Campus Großhadern, Ludwig-Maximilians University Munich (LMU), 2Partner Site Munich, Munich Heart Alliance (MHA), DZHK (German Centre for Cardiovascular Research), 3Walter Brendel Center of Experimental Medicine, Ludwig-Maximilians University Munich (LMU), 4Institute of Pharmacology and Toxicology, University Medical Center Göttingen, 5Partner Site Göttingen, DZHK (German Centre for Cardiovascular Research), 6Cluster of Excellence "Multiscale Bioimaging: from Molecular Machines to Networks of Excitable Cells" (MBExC), University of Göttingen
Rozdziały w tym wideo
0:05
Introduction
0:51
Organ Harvest
1:33
Enzymatic Digestion
3:59
Calcium Reintroduction
4:57
Results: Simultaneous Measurement of Calcium Currents and Transients in Atrial and Ventricular Murine Myocytes
5:36
Conclusion
Powiązane Filmy
14:39
Isolation and Functional Characterization of Human Ventricular Cardiomyocytes from Fresh Surgical Samples
17.2K Views
11:32
Optical Imaging of Isolated Murine Ventricular Myocytes
6.2K Views
08:19
Electrophysiological Assessment of Murine Atria with High-Resolution Optical Mapping
9.7K Views
11:33
Isolation and Kv Channel Recordings in Murine Atrial and Ventricular Cardiomyocytes
13.3K Views
10:53
Isolation of Human Atrial Myocytes for Simultaneous Measurements of Ca2+ Transients and Membrane Currents
20.3K Views
11:02
Isolation and Physiological Analysis of Mouse Cardiomyocytes
22.9K Views
08:34
Isolation of Atrial Myocytes from Adult Mice
10.3K Views
06:27
Modifications of the Langendorff Method for Simultaneous Isolation of Atrial and Ventricular Myocytes from Adult Mice
5.2K Views
10:08
Camera-based Measurements of Intracellular [Na+] in Murine Atrial Myocytes
1.2K Views
11:19
Isolation of Human Ventricular Cardiomyocytes from Vibratome-Cut Myocardial Slices
10.2K Views
Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone