Results: Simultaneous Measurement of Calcium Currents and Transients in Atrial and Ventricular Murine Myocytes
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Conclusion
副本
Patch clamp and calcium imaging experiments are labor intensive, time consuming and challenging. This protocol provides a convenient method to isolate high quality murine atrial and ventricular cardiomyocytes, suitable for patch clamp experiments
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Mouse models allow studying key mechanisms of arrhythmogenesis. For this purpose, high quality cardiomyocytes are necessary to perform patch-clamp measurements. Here, a method to isolate murine atrial and ventricular myocytes via retrograde enzyme-based Langendorff perfusion, which allows simultaneous measurements of calcium-transients and L-type calcium current, is described.