Electroporation-Based Genetic Modification of Primary Human Pigment Epithelial Cells Using the Sleeping Beauty Transposon System
Please note that all translations are automatically generated. Click here for the English version.
2.0K Views
•
07:04 min
•
February 4th, 2021
DOI :
February 4th, 2021
•Sandra Johnen1, Nina Harmening2,3, Corinne Marie4,5, Daniel Scherman4, Zsuzsanna Izsvák6, Zoltán Ivics7, Peter Walter1, Gabriele Thumann2,3
1Department of Ophthalmology, University Hospital RWTH Aachen, 2Experimental Ophthalmology, University of Geneva, 3Department of Ophthalmology, University Hospitals of Geneva, 4Université de Paris, CNRS, INSERM, UTCBS, Unité des technologies Chimiques et Biologiques pour la Santé, 5Chimie ParisTech, PSL Research University, 6Max Delbrück Center for Molecular Medicine in the Helmholtz Association, 7Division of Medical Biotechnology, Paul-Ehrlich-Institute
Przeglądaj więcej filmów
Rozdziały w tym wideo
0:05
Introduction
0:57
Primary Human RPE Cell Electroporation and Plasmid DNA Preparation
1:57
Cultivated Primary Human RPE Cell Preparation
3:19
His-Tagged Pigment Epithelium-Derived Factor (PEDF) Fusion Protein Purification
4:57
Results: Representative Primary Human RPE Transfection and Genetic Modification Evaluation
6:24
Conclusion
Powiązane Filmy
05:53
In vivo Electroporation of Developing Mouse Retina
20.8K Views
10:02
piggyBac Transposon System Modification of Primary Human T Cells
17.2K Views
09:29
Clinical Application of Sleeping Beauty and Artificial Antigen Presenting Cells to Genetically Modify T Cells from Peripheral and Umbilical Cord Blood
18.3K Views
09:03
Introducing Point Mutations into Human Pluripotent Stem Cells Using Seamless Genome Editing
4.0K Views
06:22
Neonatal Pial Surface Electroporation
13.8K Views
10:58
Transposon Mediated Integration of Plasmid DNA into the Subventricular Zone of Neonatal Mice to Generate Novel Models of Glioblastoma
12.9K Views
10:00
Induction and Analysis of Oxidative Stress in Sleeping Beauty Transposon-Transfected Human Retinal Pigment Epithelial Cells
2.5K Views
10:13
An Efficient In Vitro Transposition Method by a Transcriptionally Regulated Sleeping Beauty System Packaged into an Integration Defective Lentiviral Vector
8.9K Views
09:46
Isolation, Culture, and Genetic Engineering of Mammalian Primary Pigment Epithelial Cells for Non-Viral Gene Therapy
3.1K Views
02:09
Edycja genów za pośrednictwem transpozonów PiggyBac w ludzkich iPSC: procedura integracji genu zainteresowania w ludzkich iPSC za pomocą systemu transpozonów PiggyBac
1.3K Views
Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone