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Electroporation-Based Genetic Modification of Primary Human Pigment Epithelial Cells Using the Sleeping Beauty Transposon System

2.0K Views

07:04 min

February 4th, 2021

DOI :

10.3791/61987-v

February 4th, 2021


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Electroporation

Rozdziały w tym wideo

0:05

Introduction

0:57

Primary Human RPE Cell Electroporation and Plasmid DNA Preparation

1:57

Cultivated Primary Human RPE Cell Preparation

3:19

His-Tagged Pigment Epithelium-Derived Factor (PEDF) Fusion Protein Purification

4:57

Results: Representative Primary Human RPE Transfection and Genetic Modification Evaluation

6:24

Conclusion

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