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Electroporation-Based Genetic Modification of Primary Human Pigment Epithelial Cells Using the Sleeping Beauty Transposon System

1.9K Views

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07:04 min

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February 4th, 2021

DOI :

10.3791/61987-v

February 4th, 2021

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1,878 Views

Sandra Johnen¹, Nina Harmening²^,³, Corinne Marie⁴^,⁵, Daniel Scherman⁴, Zsuzsanna Izsvák⁶, Zoltán Ivics⁷, Peter Walter¹, Gabriele Thumann²^,³

¹Department of Ophthalmology, University Hospital RWTH Aachen, ²Experimental Ophthalmology, University of Geneva, ³Department of Ophthalmology, University Hospitals of Geneva, ⁴Université de Paris, CNRS, INSERM, UTCBS, Unité des technologies Chimiques et Biologiques pour la Santé, ⁵Chimie ParisTech, PSL Research University, ⁶Max Delbrück Center for Molecular Medicine in the Helmholtz Association, ⁷Division of Medical Biotechnology, Paul-Ehrlich-Institute

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Electroporation

Sleeping Beauty Transposon System

Primary Human Pigment Epithelial Cells

Transgene Expression

Protein Secretion

Retinal Degenerative Diseases

Gene Addition Therapy

Plasmid DNA

Transposase

Pigment Epithelium Derived Factor

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