Primary Human RPE Cell Electroporation and Plasmid DNA Preparation
1:57
Cultivated Primary Human RPE Cell Preparation
3:19
His-Tagged Pigment Epithelium-Derived Factor (PEDF) Fusion Protein Purification
4:57
Results: Representative Primary Human RPE Transfection and Genetic Modification Evaluation
6:24
Conclusion
필기록
This method demonstrates electroporation-based transfection of primary human pigment epithelial cells using the Sleeping Beauty Transposon System, an evidence for transgene expression and protein secretion. The combination of the Sleeping Beauty T
이 콘텐츠에 액세스하려면 로그인하거나 무료 평가판을 시작하세요
We have developed a protocol to transfect primary human pigment epithelial cells by electroporation with the gene encoding pigment epithelium-derived factor (PEDF) using the Sleeping Beauty (SB) transposon system. Successful transfection was demonstrated by quantitative polymerase chain reaction (qPCR), immunoblotting, and enzyme-linked immunosorbent assay (ELISA).