An Economical and Versatile High-Throughput Protein Purification System Using a Multi-Column Plate Adapter

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4.1K Views

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10:08 min

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May 21st, 2021

DOI :

10.3791/62075-v

May 21st, 2021

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Florence B Kineavy¹, Alex A Davies¹, Molly R Mitchell¹, Daniel Lay¹, Matt J Dominguez², Elliott J Stollar¹

¹School of Life Sciences, University of Liverpool, ²Health Sciences Center, Texas Tech University

Podsumowanie

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High throughput Protein Purification

Multi column Plate Adapter

Protein Purification Method

Nickel Affinity Chromatography

Ion Exchange Chromatography

MCPA

Biophysical Techniques

Functional Proteomics

Chromatography Columns

Resin Volumes

EDTA Buffer

Sodium Hydroxide Buffer

Nickel Sulfate

Imidazole Wash Buffer

Rozdziały w tym wideo

0:00

Introduction

0:58

Ni-NTA Chromatography- Purification of 24 Different Proteins: Preparing the Purification Set-up

3:00

Ni-NTA Chromatography- Purification of 24 Different Proteins: Loading, Washing and Eluting

4:47

Ion Exchange- Simultaneous Purification of 6 Different Proteins: Preparing the Purification Set-up

6:15

Ion Exchange- Simultaneous Purification of 6 Different Proteins: Loading, Washing and Eluting

8:25

Results: Representative UV Spectroscopy and SDS PAGE Analyses

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