Ni-NTA Chromatography- Purification of 24 Different Proteins: Preparing the Purification Set-up
3:00
Ni-NTA Chromatography- Purification of 24 Different Proteins: Loading, Washing and Eluting
4:47
Ion Exchange- Simultaneous Purification of 6 Different Proteins: Preparing the Purification Set-up
6:15
Ion Exchange- Simultaneous Purification of 6 Different Proteins: Loading, Washing and Eluting
8:25
Results: Representative UV Spectroscopy and SDS PAGE Analyses
Transkript
An economical and versatile high throughput protein purification system using a multi column plate adapter. Hi, protein purification is imperative to the study of protein structure and function. And it's usually used in combination with biophysica
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A multi-column plate adapter allows chromatography columns to be interfaced with multi-well collection plates for parallel affinity or ion exchange purification providing an economical high throughput protein purification method. It can be used under gravity or vacuum yielding milligram quantities of protein via affordable instrumentation.