Staining and High-Resolution Imaging of Three-Dimensional Organoid and Spheroid Models

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11.4K Views

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07:35 min

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March 27th, 2021

DOI :

10.3791/62280-v

March 27th, 2021

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Alejandro Lopez Gonzalez*¹, Léa Luciana*¹, Clémentine Le Nevé², Julie Valantin², Laura Francols², Nicolas Gadot², Christophe Vanbelle³, Laurianne Davignon⁴, Laura Broutier¹

¹Childhood Cancer & Cell Death (C3), Université de Lyon, Université Claude Bernard Lyon 1, INSERM 1052, CNRS 5286, Centre Léon Bérard, Centre de recherche en cancérologie de Lyon (CRCL), Lyon 69373, ²Plateforme Anatomopathologie Recherche, Université de Lyon, Université Claude Bernard Lyon 1, INSERM 1052, CNRS 5286, Centre Léon Bérard, Centre de recherche en cancérologie de Lyon (CRCL), ³Plateforme d’Imagerie cellulaire, Université de Lyon, Université Claude Bernard Lyon 1, INSERM 1052, CNRS 5286, Centre Léon Bérard, Centre de recherche en cancérologie de Lyon (CRCL), ⁴PerkinElmer S.A.S.

Podsumowanie

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3D Organoid

3D Modeling

Staining Protocol

High resolution Imaging

Subcellular Resolution

Pipetting Technique

Permeabilization Blocking Solution

Cellular Visualization

Microscopy Technique

Lab Procedures

Pathology Research Platform

Rozdziały w tym wideo

0:04

Introduction

1:05

3D Whole Mount Staining

3:09

Embedding of Large 3D Cell Culture Models

5:19

Embedding of Small 3D Cell Culture Models

6:26

Results: High‐Resolution Imaging of 3D Structures and Spheroid Models

7:00

Conclusion

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