Staining and High-Resolution Imaging of Three-Dimensional Organoid and Spheroid Models
11.5K Views
•
07:35 min
•
March 27th, 2021
DOI :
March 27th, 2021
•Alejandro Lopez Gonzalez*1, Léa Luciana*1, Clémentine Le Nevé2, Julie Valantin2, Laura Francols2, Nicolas Gadot2, Christophe Vanbelle3, Laurianne Davignon4, Laura Broutier1
1Childhood Cancer & Cell Death (C3), Université de Lyon, Université Claude Bernard Lyon 1, INSERM 1052, CNRS 5286, Centre Léon Bérard, Centre de recherche en cancérologie de Lyon (CRCL), Lyon 69373, 2Plateforme Anatomopathologie Recherche, Université de Lyon, Université Claude Bernard Lyon 1, INSERM 1052, CNRS 5286, Centre Léon Bérard, Centre de recherche en cancérologie de Lyon (CRCL), 3Plateforme d’Imagerie cellulaire, Université de Lyon, Université Claude Bernard Lyon 1, INSERM 1052, CNRS 5286, Centre Léon Bérard, Centre de recherche en cancérologie de Lyon (CRCL), 4PerkinElmer S.A.S.
Chapters in this video
0:04
Introduction
1:05
3D Whole Mount Staining
3:09
Embedding of Large 3D Cell Culture Models
5:19
Embedding of Small 3D Cell Culture Models
6:26
Results: High‐Resolution Imaging of 3D Structures and Spheroid Models
7:00
Conclusion
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